3D Visualization for Novel Insights into Brain Structure

3D 可视化对大脑结构的新见解

• 批准号: 8360019
• 负责人: GEORGE A SPIROU
• 金额: $ 22.45万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360019
• 关键词: 3-Dimensional; Auditory; Auditory system; Axon; Biological; Biology; Blood Vessels; Blood capillaries; Brain; Brain Pathology; California; Cell Nucleus; Cell Polarity; Cells; Cellular Structures; Cochlear nucleus; Cognitive; Collection; Complex; Computer software; Data; Data Collection; Data Display; Data Set; Dimensions; Electron Microscopy; Elements; Environment; Exhibits; Face; Fascicle; Fiber; Goals; Grouping; Human; Image; Imagery; Institution; Investigation; Link; Maps; Medial; Metabolic; Metric; National Institute for Occupational Safety and Health; Nerve Fibers; Nervous system structure; Neuroglia; Neurons; Organelles; Pattern; Procedures; Process; Property; Publishing; Relative (related person); Reporting; Research Personnel; Resolution; Scanning Electron Microscopy; Speed; Structure; Subcellular structure; Synapses; System; Techniques; Technology; Therapeutic; Thick; Tissues; Universities; Vascular blood supply; Visual; West Virginia; abstracting; base; brain tissue; capillary; experience; information processing; insight; instrument; interest; new technology; novel; relating to nervous system; spatial relationship; trapezoid body; virtual reality

DESCRIPTION (provided by applicant): The nervous system is a complex arrangement of neurons, their axonal projections, glia and vascular supply, yet our concepts about information processing are heavily neuron-centric. This bias results from the efficiency of standard anatomical techniques to map synaptic connections between neurons relative to other physical interactions among neural and non-neural cells. Spatial organization of neuronal groups and glia networks is indicated in many studies, but spatial organization and interactions have not been studied with an integrated approach. We will employ a new technology, serial block-face scanning electron microscopy, to reconstruct, at high resolution, all cellular elements of large tissue volumes to identify new principles in nervous system cellular organization. We will tap into human visual-cognitive capabilities to understand spatial order by developing procedures, implemented in a software user interface, to display combinations of cellular elements and vascular structures in 3-D virtual reality environments. Identification of structural features that define 3D organization of tissue will facilitate investigation of large-scale tissue volumes by othr investigators as high-throughput, high resolution studies of brain structure become more common. PUBLIC HEALTH RELEVANCE: The goal of this project is to reveal new principles of tissue organization in the brain. We expect that our experimental results will provide a basis for new metrics to evaluate brain pathology and therapeutic strategies.

描述（申请人提供）：神经系统是一个复杂的神经元排列，它们的轴突投射，胶质和血管供应，但我们关于信息处理的概念很大程度上是以神经元为中心的。这种偏差源于标准解剖技术在绘制神经元之间的突触连接相对于神经细胞和非神经细胞之间的其他物理相互作用时的效率。许多研究表明神经元群和神经胶质网络的空间组织，但空间组织和相互作用尚未得到综合研究。我们将采用一种新技术，连续块面扫描电子显微镜，以高分辨率重建大组织体积的所有细胞元素，以确定神经系统细胞组织的新原理。我们将利用人类的视觉认知能力，通过开发程序来理解空间秩序，在软件用户界面中实现，在三维虚拟现实环境中显示细胞元素和血管结构的组合。结构特征的识别