3D Visualization for Novel Insights into Brain Structure

3D 可视化对大脑结构的新见解

• 批准号: 8360019
• 负责人: GEORGE A SPIROU
• 金额: $ 22.45万
• 依托单位: WEST VIRGINIA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8360019
• 关键词: 3-Dimensional; Auditory; Auditory system; Axon; Biological; Biology; Blood Vessels; Blood capillaries; Brain; Brain Pathology; California; Cell Nucleus; Cell Polarity; Cells; Cellular Structures; Cochlear nucleus; Cognitive; Collection; Complex; Computer software; Data; Data Collection; Data Display; Data Set; Dimensions; Electron Microscopy; Elements; Environment; Exhibits; Face; Fascicle; Fiber; Goals; Grouping; Human; Image; Imagery; Institution; Investigation; Link; Maps; Medial; Metabolic; Metric; National Institute for Occupational Safety and Health; Nerve Fibers; Nervous system structure; Neuroglia; Neurons; Organelles; Pattern; Procedures; Process; Property; Publishing; Relative (related person); Reporting; Research Personnel; Resolution; Scanning Electron Microscopy; Speed; Structure; Subcellular structure; Synapses; System; Techniques; Technology; Therapeutic; Thick; Tissues; Universities; Vascular blood supply; Visual; West Virginia; abstracting; base; brain tissue; capillary; experience; information processing; insight; instrument; interest; new technology; novel; relating to nervous system; spatial relationship; trapezoid body; virtual reality

DESCRIPTION (provided by applicant): The nervous system is a complex arrangement of neurons, their axonal projections, glia and vascular supply, yet our concepts about information processing are heavily neuron-centric. This bias results from the efficiency of standard anatomical techniques to map synaptic connections between neurons relative to other physical interactions among neural and non-neural cells. Spatial organization of neuronal groups and glia networks is indicated in many studies, but spatial organization and interactions have not been studied with an integrated approach. We will employ a new technology, serial block-face scanning electron microscopy, to reconstruct, at high resolution, all cellular elements of large tissue volumes to identify new principles in nervous system cellular organization. We will tap into human visual-cognitive capabilities to understand spatial order by developing procedures, implemented in a software user interface, to display combinations of cellular elements and vascular structures in 3-D virtual reality environments. Identification of structural features that define 3D organization of tissue will facilitate investigation of large-scale tissue volumes by othr investigators as high-throughput, high resolution studies of brain structure become more common. PUBLIC HEALTH RELEVANCE: The goal of this project is to reveal new principles of tissue organization in the brain. We expect that our experimental results will provide a basis for new metrics to evaluate brain pathology and therapeutic strategies.

描述（由申请人提供）：神经系统是神经元的复杂布置，它们的轴突预测，神经胶质和血管供应，但是我们关于信息处理的概念以神经元为中心。这一偏见是由于标准解剖技术绘制神经元之间相对于神经和非神经细胞之间其他物理相互作用的突触连接的效率所致。许多研究都表明了神经元组和神经网络的空间组织，但是空间组织和相互作用尚未通过综合方法研究。我们将采用一种新技术，即串行块扫描电子显微镜，以高分辨率重建大型组织量的所有细胞元素，以识别神经系统细胞组织中的新原理。我们将利用人类的视觉认知能力来通过开发在软件用户界面中实现的过程来理解空间顺序，以在3-D虚拟现实环境中显示蜂窝元素和血管结构的组合。识别结构特征 定义3D组织的组织将促进OTHR研究者的大规模组织体积的研究，因为高通量，大脑结构的高分辨率研究变得更加普遍。 公共卫生相关性：该项目的目的是揭示大脑中组织组织的新原则。我们预计我们的实验结果将为新指标评估脑病理学和治疗策略提供基础。