COMBINED LASER AND TOPICAL ANTIANGIOGENIC THERAPY OF PORT WINE STAIN

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 与激光照射皮肤中的伤口愈合反应相关的血管生成可引起光凝血管的再生，从而降低血管病变（例如葡萄酒色斑胎记）的治疗结果。我们的具体目标是确定激光和局部雷帕霉素的联合使用是否会诱导长期血管切除动物模型。将使用多个激光脉冲照射啮齿动物窗室模型（RWCM）中的血管，以确保可靠和密集的光凝。将通过彩色数字摄影和激光散斑成像记录血管的进展。一组RWCM将仅用激光照射，另一组RWCM将用激光照射，并将抗血管生成剂雷帕霉素（RPM）应用于表皮侧14天。我们将比较这两组中光凝血管的再生，以确定局部雷帕霉素的效果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Angiogenesis associated with the wound healing response in laser-irradiated skin can cause regeneration of the photocoagulated blood vessels and thus reduce the therapeutic outcome of vascular lesions such as port wine stain birthmarks. Our specific aim is to determine whether the combined use of laser and topical rapamycin will induce long-term blood vessel removal using animal models. Blood vessels in the rodent window chamber model (RWCM) will be irradiated with multiple laser pulses to ensure reliable and intensive photocoagulation. The progression of the blood vessels will be recorded by color digital photography and laser speckle imaging. One group of RWCM will be irradiated with laser only and another group of RWCM will be irradiated with laser and an anti-angiogenic agent, rapamycin (RPM) will be applied to the epidermal side for 14 days. We will compare the regrowth of photocoagulated blood vessels in these two group to determine the effect of topical rapamycin.