COMBINED LASER AND TOPICAL ANTIANGIOGENIC THERAPY OF PORT WINE STAIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在激光照射的皮肤中，与伤口愈合反应相关的血管生成可以导致光凝血管的再生，从而降低血管病变的治疗结果，如鲜红斑点胎记。我们的具体目标是通过动物模型确定联合使用激光和外用雷帕霉素是否会导致长期的血管切除。啮齿动物窗口室模型(RWCM)中的血管将被多个激光脉冲照射，以确保可靠和密集的光凝。血管的进展将通过彩色数字摄影和激光散斑成像来记录。一组RWCM仅用激光照射，另一组RWCM用激光照射，并用抗血管生成剂雷帕霉素(RPM)涂于表皮侧，为期14天。我们将比较这两组光凝血管的再生情况，以确定外用雷帕霉素的效果。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Angiogenesis associated with the wound healing response in laser-irradiated skin can cause regeneration of the photocoagulated blood vessels and thus reduce the therapeutic outcome of vascular lesions such as port wine stain birthmarks. Our specific aim is to determine whether the combined use of laser and topical rapamycin will induce long-term blood vessel removal using animal models. Blood vessels in the rodent window chamber model (RWCM) will be irradiated with multiple laser pulses to ensure reliable and intensive photocoagulation. The progression of the blood vessels will be recorded by color digital photography and laser speckle imaging. One group of RWCM will be irradiated with laser only and another group of RWCM will be irradiated with laser and an anti-angiogenic agent, rapamycin (RPM) will be applied to the epidermal side for 14 days. We will compare the regrowth of photocoagulated blood vessels in these two group to determine the effect of topical rapamycin.