INDOCYANINE GREEN PHOTODYNAMIC THERAPY FOR VASCULAR LESIONS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的实验旨在评估ICG-PDT对小鼠窗室血管系统（模拟PWS血管系统的动物模型）的作用。 具体而言，我们感兴趣的是确定ICG-PDT实现的血管关闭水平。 小鼠将接受ICG，然后接受激光照射。 ICG将通过眶后注射，使ICG扩散到血流中。 延迟1分钟后，用GentleLASE 755 nm紫翠宝石激光照射皮褶的表皮侧;脉冲持续时间和光斑大小分别为3 ms和10 mm。 然后将小鼠暴露于30、40或50 J/cm 2的辐射暴露。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our experiments aim to evaluate the effect of ICG-PDT on mouse window chamber vasculature (an animal model which simulates PWS vasculature). Specifically, we are interested in determining the level of vascular shutdown achieved with ICG-PDT. Mice will receive ICG followed by laser irradiation. ICG will be injected retroorbitally allowing the ICG to diffuse into the bloodstream. Following a 1-minute delay, the epidermal side of the skin-fold will be irradiated with a GentleLASE 755 nm alexandrite laser; pulse duration and spot size will be 3 ms and 10 mm respectively. Mice will then be exposed to radiant exposures of 30, 40 or 50 J/cm2.