AXEL BRUNGER PRT TIME

阿克塞尔·布伦格 PRT 时间

• 批准号: 8362040
• 负责人: AXEL T BRUNGER
• 金额: $ 1.26万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362040
• 关键词: Collection; Complex; Dimensions; Dyes; Face; Funding; Gentian Violet; Goals; Grant; Measures; National Center for Research Resources; Principal Investigator; Property; Proteins; Radiation; Research; Research Infrastructure; Resources; Source; Staining method; Stains; Twin Multiple Birth; United States National Institutes of Health; cost; research study; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The proposed experiments involve the characterization of small protein crystals. The protein in question is a fairly large (~500 kDa) complex of two different proteins. The crystals stain blue in the presence of crystal violet dye, indicating that they are composed of protein. However, the crystals grow only as small plates, with dimensions of approximately 100-200 microns on the plate face and 50 microns measured orthogonal to the face. The crystals are to be studied to determine extent of diffraction, twinning and mosaicity. Potential improvements in cryo-conditions will also be examined. The crystals have been grown in a variety of different substrates. The effect of these substrates upon the diffraction properties will be studied as well. Determination of space group and the collection of a native set for these crystals would be the ultimate goal.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 拟议的实验涉及小蛋白质晶体的表征。 所讨论的蛋白质是两种不同蛋白质的相当大（~500 kDa）的复合物。 晶体在结晶紫染料的存在下染成蓝色，表明它们由蛋白质组成。 然而，晶体仅生长为小板，板面上的尺寸约为100-200微米，垂直于板面测量的尺寸为50微米。对晶体进行研究，以确定衍射、孪生和镶嵌的程度。 还将研究低温条件的潜在改进。 晶体已经在各种不同的衬底中生长。 这些基板上的衍射性能的影响也将进行研究。 空间群的确定和这些晶体的本地集合的收集将是最终目标。