STRUCTURAL AND FUNCTIONAL STUDIES OF P97
P97的结构和功能研究
基本信息
- 批准号:7721770
- 负责人:
- 金额:$ 0.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2008
- 资助国家:美国
- 起止时间:2008-03-01 至 2009-02-28
- 项目状态:已结题
- 来源:
- 关键词:ApoptosisArchaeaBiologicalCell NucleusComplexComputer Retrieval of Information on Scientific Projects DatabaseDNA biosynthesisDataData SetDegradation PathwayDistantEndoplasmic ReticulumFundingFutureGolgi ApparatusGrantGrowthHomologous GeneHydrolysisIndividualInstitutionKLK3 geneMembrane FusionMembrane ProteinsNucleotidesResearchResearch PersonnelResourcesRoleSourceStructureUbiquitinUnited States National Institutes of HealthYeastsadapter proteinluminal membranevalosin-containing protein
项目摘要
This subproject is one of many research subprojects utilizing the
resources provided by a Center grant funded by NIH/NCRR. The subproject and
investigator (PI) may have received primary funding from another NIH source,
and thus could be represented in other CRISP entries. The institution listed is
for the Center, which is not necessarily the institution for the investigator.
We are pursuing structural and functional studies of p97 (also called VCP, valosin-containing protein; VAT in Archaebacteria; and cdc48 in yeast), a distant homolog of NSF. p97 has been implicated in postmitotic homotypic membrane fusion of the endoplasmic reticulum (ER) and of the Golgi apparatus, assembly and growth of the nucleus, apoptosis, and DNA replication. It is also involved in the ubiquitin-dependent degradation pathway, as well as the extraction of misfolded luminal and membrane proteins from the ER for cytosolic degradation. We recently determined the crystal structure of the biologically complete p97/VCP hexamer complexed with a mixture of ADP and ADP.AlF(x)using diffraction data collected at SSRL and APS. The MAD dataset collected at SSRL provided the key to solve the structure. The structure revealed the quarternary configuration of the individual domains and the connecting linkers. Our crystal structure represents a snapshot of p97/VCP as it proceeds through its nucleotide hydrolysis cycle. Our future plans include structural studies of complexes involving adapter proteins and substrates for p97/VCP, to elucidate the mechanism and biological role of p97/VCP.
这个子项目是许多研究子项目中的一个
由NIH/NCRR资助的中心赠款提供的资源。子项目和
研究者(PI)可能从另一个NIH来源获得了主要资金,
因此可以在其他CRISP条目中表示。列出的机构为
中心,但不一定是研究者所在的机构。
我们正在进行p97(也称为VCP,含缬草根素的蛋白质;古细菌中的增值税;和酵母中的cdc 48)的结构和功能研究,它是NSF的远端同源物。p97与内质网(ER)和高尔基体的有丝分裂后同型膜融合、细胞核的组装和生长、细胞凋亡和DNA复制有关。它还参与泛素依赖性降解途径,以及从ER中提取错误折叠的管腔和膜蛋白用于胞质降解。最近,我们使用在SSRL和APS收集的衍射数据确定了与ADP和ADP·AlF(x)的混合物复合的生物学上完整的p97/VCP六聚体的晶体结构。在SSRL收集的MAD数据集提供了解决结构的关键。 结构揭示了各个结构域和连接接头的四元构型。我们的晶体结构代表了p97/VCP在其核苷酸水解循环中的快照。我们未来的计划包括研究p97/VCP的接头蛋白和底物的复合物的结构,以阐明p97/VCP的机制和生物学作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('AXEL T BRUNGER', 18)}}的其他基金
MECHANISM OF BOTULINUM NEUROTOXIN TARGET, SUBSTRATE, AND INHIBITOR INTERACTIONS
肉毒杆菌神经毒素靶标、底物和抑制剂相互作用的机制
- 批准号:
8362050 - 财政年份:2011
- 资助金额:
$ 0.44万 - 项目类别:
MECHANISM OF BOTULINUM NEUROTOXIN TARGET, SUBSTRATE, AND INHIBITOR INTERACTIONS
肉毒杆菌神经毒素靶标、底物和抑制剂相互作用的机制
- 批准号:
8169924 - 财政年份:2010
- 资助金额:
$ 0.44万 - 项目类别:
MECHANISM OF BOTULINUM NEUROTOXIN TARGET, SUBSTRATE, AND INHIBITOR INTERACTIONS
肉毒杆菌神经毒素靶标、底物和抑制剂相互作用的机制
- 批准号:
7954183 - 财政年份:2009
- 资助金额:
$ 0.44万 - 项目类别:
STRUCTURAL & FUNCTIONAL STUDIES OF HUMAN VESICULAR NEUROTRANSMITTER TRANSPORTERS
结构性
- 批准号:
7955126 - 财政年份:2009
- 资助金额:
$ 0.44万 - 项目类别:
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