STRUCT STUD OF MEMBRANE & PROTEIN COMPLEX INVOLVED IN ENERGY CONV & SIGNAL

膜结构螺柱

• 批准号: 8363524
• 负责人: EDWARD Alan BERRY
• 金额: $ 0.57万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363524
• 关键词: Affect; Agriculture; Binding; Chemicals; Complex; Cytochrome bc1 Complex; Development; Electron Transport; Funding; Grant; Industrial fungicide; Membrane Proteins; National Center for Research Resources; Natural Immunity; Pharmacologic Substance; Positioning Attribute; Principal Investigator; Research; Research Infrastructure; Resources; Rieske iron-sulfur protein; Signal Transduction; Source; Testing; Time; United States National Institutes of Health; atovaquone; cost; enzyme mechanism; inhibitor/antagonist; protein complex; respiratory

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The cytochrome bc1 complex is an important target for pharmaceutical (e.g. Atovaquone, Leucascandrolide) and agricultural fungicides (azoxystrobind, kresoxym-methyl, famoxadone) These chemical affect the position of the Rieske iron-sulfur protein (RISP) in the crystals in a fashion that may reflect a catalytic switch mechanism for gating the electron transfer. We are studying the mode of binding of these inhibitors to elucidate the mechanism of the enzyme and to enable development of better inhibitors. Some of the time will also be used for testing new crstals from projects related to respiratory complex maturation, innate immunity, and signal transduction.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 细胞色素 bc1 复合物是药物（例如 Atovaquone、Leucascandrolide）和农业杀菌剂（嘧菌酯、醚菌酯、恶唑酮）的重要靶标。这些化学物质影响 Rieske 铁硫蛋白 (RISP) 在晶体中的位置，其方式可能反映了门控电子转移的催化开关机制。我们正在研究这些抑制剂的结合模式，以阐明酶的机制并开发更好的抑制剂。 部分时间还将用于测试与呼吸复合体成熟、先天免疫和信号转导相关的项目中的新晶体。