MAD DIFFRACTION DATA COLLECTION FROM CYTOCHROME REDUCTASE CRYSTALS

细胞色素还原酶是一种大分子膜蛋白复合体。 包括线粒体呼吸链的中间部分。 我们收集了由X射线确定的3个波长的MAD数据集 晶体的吸收光谱。当与DM分阶段时-改进 同构相，这个数据集清楚地定位了铁的中心在 水晶。从数据计算出的相位低于 同构的阶段，但数据被用作独立的 监测特定分辨率中的相的好坏的标准 阶段改进/扩展期间的壳，峰值高度为 与测试阶段同步的异常差值图。

Cytochrome reductase is a large membrane protein complex which comprises the middle section of the mitochondrial respiratory chain. We collected a MAD dataset at 3 wavelengths determined by an X-ray absorption spectrum of the crystals. When phased with DM-improved isomorphous phases, this dataset clearly located the iron centers in the crystal. Phases calculated from the data are inferior to the isomorphous phases, but the data is being used as an independent criterion to monitor goodness of phases in particular resolution shells during phase improvement/extension by the height of peaks in anomalous difference maps phased with the test phases.