MAD DIFFRACTION DATA COLLECTION FROM CYTOCHROME REDUCTASE CRYSTALS

细胞色素还原酶晶体的疯狂衍射数据收集

• 批准号: 6586518
• 负责人: EDWARD Alan BERRY
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586518
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology; technology /technique

Cytochrome reductase is a large membrane protein complex which comprises the middle section of the mitochondrial respiratory chain. We collected a MAD dataset at 3 wavelengths determined by an X-ray absorption spectrum of the crystals. When phased with DM-improved isomorphous phases, this dataset clearly located the iron centers in the crystal. Phases calculated from the data are inferior to the isomorphous phases, but the data is being used as an independent criterion to monitor goodness of phases in particular resolution shells during phase improvement/extension by the height of peaks in anomalous difference maps phased with the test phases.

细胞色素还原酶是一种大的膜蛋白复合物， 包括线粒体呼吸链的中段。 我们收集了由X射线确定的3个波长的MAD数据集 晶体的吸收光谱。 当与DM-改进分阶段时 同晶相，该数据集清楚地定位了铁中心， 水晶 根据数据计算的相位劣于 同构相，但数据被用作独立的 在特定分辨率下监测相位良好性的准则 在相位改善/扩展期间， 与测试阶段同阶段的异常差异图。