XRAY DIFFRACTION INVESTIGATION OF CYTOCHROME REDUCTASE CRYSTALS

细胞色素还原酶晶体的 X 射线衍射研究

• 批准号: 6586524
• 负责人: EDWARD Alan BERRY
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586524
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; enzymes; radiography; structural biology

Cytochrome reductase is a large membrane protein complex which comprises the middle section of the mitochondrial respiratory chain. In 1994 we improved the crystals and tested protein from different species. Rabbit reductase crystallized in the same hexagonal space group with significantly improved order. Porcine reductase crystallized in a new space group with poor order. Chicken reductase gave orthorhombic (P212121) crystals with markedly better order, diffracting to 3 E. Furthermore these crystals can be frozen with no loss of order after equilibrating with cryoprotectant solution. We have located heavy atom sites in a TAMM derivative of the crystal.

细胞色素还原酶是一种大的膜蛋白复合物， 包括线粒体呼吸链的中段。 1994年，我们改进了晶体，并测试了不同蛋白质的含量。 物种 兔还原酶在同一个六角形空间结晶 秩序明显改善。 猪还原酶 在新的空间群中结晶，有序性差。 鸡还原酶 得到了具有明显更好有序性的斜方晶体（P212121）， 衍射到3E。此外，这些晶体可以冷冻， 与冷冻保护剂溶液平衡后失去秩序。 我们 已经在晶体的TAMM衍生物中定位了重原子位点。