XRAY DIFFRACTION INVESTIGATION OF CYTOCHROME REDUCTASE CRYSTALS

细胞色素还原酶是一种大分子膜蛋白复合体。 包括线粒体呼吸链的中间部分。 1994年，我们改进了晶体，并测试了不同来源的蛋白质。 物种。兔还原酶在相同的六方空间中结晶 秩序有了显著改善的小组。猪还原酶 结晶在一个新的空间群中，有序性差。鸡肉还原酶 得到了有序度明显提高的斜方晶系(P212121)晶体， 衍射到3E。此外，这些晶体可以用NO冷冻。 与低温保护剂溶液平衡后失去有序性。我们 已经在晶体的Tamm衍生物中找到了重原子位置。

Cytochrome reductase is a large membrane protein complex which comprises the middle section of the mitochondrial respiratory chain. In 1994 we improved the crystals and tested protein from different species. Rabbit reductase crystallized in the same hexagonal space group with significantly improved order. Porcine reductase crystallized in a new space group with poor order. Chicken reductase gave orthorhombic (P212121) crystals with markedly better order, diffracting to 3 E. Furthermore these crystals can be frozen with no loss of order after equilibrating with cryoprotectant solution. We have located heavy atom sites in a TAMM derivative of the crystal.