XRAY DIFFRACTION INVESTIGATION OF CYTOCHROME REDUCTASE

细胞色素还原酶的 X 射线衍射研究

• 批准号: 6586584
• 负责人: EDWARD Alan BERRY
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586584
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; enzymes; radiography; structural biology

Cytochrome reductase is the middle section of the mitochondrial respiratory chain, passing electrons between the dehydrogenases and cytochrome oxidase and conserving energy in the form of an electrochemical proton gradient across the mitochondrial membrane. We have collected data to 4.5 E (complete to about 5 E) on heavy atom derivatives of the hexagonal crystal produced by co-crystallizing with a tightly-binding synthetic inhibitor containing a mercury atom. We have located a heavy atom site in the TAMM derivative of beef crystals. We have developed a freezing protocol for the beef crystals and crystallized the enzyme from 3 new sources, two of which are very promising. We need SR for more screening and data collection.

细胞色素还原酶是线粒体的中段 呼吸链，在呼吸酶之间传递电子， 细胞色素氧化酶和保存能量的形式， 线粒体膜上的电化学质子梯度 我们 收集了4.5E（约5E）的重原子数据 通过共结晶产生的六方晶体的衍生物， 一种含有汞原子的合成抑制剂。 我们 已经在牛肉的TAMM衍生物中定位了一个重原子位点 晶体 我们已经制定了牛肉晶体的冷冻程序 并从3个新的来源结晶酶，其中两个非常 很有希望 我们需要SR进行更多的筛选和数据收集。