SYNTHESIS OF SMALL MOLECULES TO PROBE ENZYMATIC FUNCTION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们的实验室对甲基标记作为蛋白质和核酸功能的调节剂感兴趣。在核小体的背景下，我们对组蛋白去甲基酶的活性、调节和化学抑制感兴趣，组蛋白去甲基酶是参与转录调控的关键酶。此外，我们正在研究核糖体RNA甲基化在翻译中的作用。为了这些项目的成功，准确地测量底物大分子的相对分子质量，并通过分析碎片模式来确定准确的修饰位置是至关重要的。因此，如果没有质谱学的关键投入，这些项目是不可能完成的。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our lab in interested in methyl marks as modulators of protein and nucleic acid function. In the context of nucleosomes, we are interested in activity, regulation and chemical inhibition of histone demethylases, key enzymes involved in regulation of transcription. Additionally, we are investigating role of methylation of ribosomal RNA in translation. For success of these projects, it is crucial to accurately measure molecular weight of substrate macromolecules, and identify, via analysis of fragmentation patterns, exact sites of modification. Thus, these projects can not be accomplished without key inputs from mass spectrometry.