SYNTHESIS OF SMALL MOLECULES TO PROBE ENZYMATIC FUNCTION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 我们的实验室对化学工具的开发感兴趣，以研究与以下相关的过程：1.染色质修饰和2.碳水化合物在内质网中的作用。 为此，我们需要进行以下综合：1.非天然氨基酸和掺入它们的短肽-用于组蛋白脱甲基酶的基于机制的失活; 2.碳水化合物，从单糖到寡糖-用于研究碳水化合物在糖蛋白生物合成和降解中的作用。 合成中间体的分子量数据对于合成中间体的表征是必要的。此外，通过质谱法评价了候选机制灭活剂对脱甲基酶的修饰位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Our lab is interested in the development of chemical tools to study processes related to: 1. chromatin modifications and 2. the role of carbohydrates in the endoplasmic reticulum. For this purpose, we need to carry out the following syntheses: 1. unnatural amino acids and short peptides incorporating them - for mechanism-based inactivation of histone demethylases; 2. carbohydrates, from monosaccharides to oligosaccharides - for studies on the role of carbohydrates in biosynthesis and degradation of glycoproteins. Data on the molecular weight of synthetic intermediates is necessary for the characterization of synthetic intermediates. Furthermore, site of modification of the demethylase by candidate mechanism-based inactivators is evaluated by mass spectrometry.