MEASURING LIGAMENT FIBER ORIENTATION USING SHG

使用 SHG 测量韧带纤维方向

• 批准号: 8362577
• 负责人: Beth A Winkelstein
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362577
• 关键词: Birefringence; Collagen; Collagen Fiber; Data; Fiber; Funding; Generations; Grant; Image; Injury; Laboratories; Ligaments; Link; Measures; Mechanics; Methods; Modeling; National Center for Research Resources; Neck Injuries; Neurologic; Optics; Pain; Preparation; Principal Investigator; Process; Research; Research Infrastructure; Resources; Sampling; Simulate; Source; Staging; Stretching; Surface; Techniques; Technology; Tissues; United States National Institutes of Health; Vertebral column; Whiplash Injuries; base; chronic pain; cost; kinematics; light transmission; polarized light; second harmonic

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our lab focuses on studying neck injuries that produce chronic pain, and part of this research has recently incorporated imaging studies of ligaments in the spine. We believe that changes to the fiber microstructure may be an important component in producing chronic pain following injury. Previously, we have used polarized light to observe the changes in collagen fiber microstructure as the ligament is stretched, simulating a whiplash injury. This technique is based on transmission of light through the tissue, requiring destructive sample preparation. We believe that surface-based methods, like second harmonic generation, would be a good next step for our injury model. We would like to develop polarization-sensitive second harmonic generation imaging and utilize the birefringence of the collagen network, which will allow us to observe changes to the network following injurious loading. While we are still in preliminary stages, if this technology provides relevant data, it may prove an important part of our injury model, linking fiber kinematics to other mechanical and neurologic pain markers.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 我们的实验室专注于研究导致慢性疼痛的颈部损伤，这项研究的一部分最近纳入了脊柱韧带的成像研究。我们认为，纤维微观结构的变化可能是损伤后产生慢性疼痛的重要组成部分。在此之前，我们已经使用偏振光观察了胶原纤维微结构的变化，因为韧带被拉伸，模拟挥鞭样损伤。该技术基于光通过组织的传输，需要破坏性的样品制备。我们相信基于表面的方法，如二次谐波产生，将是我们损伤模型的下一步。我们希望开发偏振敏感的二次谐波产生成像，并利用胶原蛋白网络的双折射，这将使我们能够观察有害负载后网络的变化。虽然我们仍处于初步阶段，但如果这项技术提供了相关数据，它可能会成为我们损伤模型的重要组成部分，将纤维运动学与其他机械和神经疼痛标志物联系起来。