VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们的目标是确定调节委内瑞拉马脑炎病毒(VEEV)和一种高度发达的生物武器制剂在没有有效治疗的情况下协同组装的分子间相互作用。VEEV是一种在中南美洲引起流行病的甲型病毒。目的1.利用互补的生化、生物物理、分子和结构生物学方法，确定VEE病毒衣壳蛋白与促进病毒核衣壳组装的RNA之间的相互作用。目的2.通过测定病毒前、病毒和病毒后核衣壳的结构，确定病毒核衣壳内的分子间衣壳蛋白相互作用在VEE病毒成熟过程中是否发生大规模重排。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our goal is to determine intermolecular interactions that regulate the concerted assembly of Venezuelan equine encephalitis virus (VEEV), an alphavirus that causes epidemics in Central and South America, and a highly developed biological weapons agent without effective therapy. Aim 1. To determine interactions between the VEE virus capsid protein and RNA that promote viral nucleocapsid assembly, using complementary biochemical, biophysical, molecular and structural biology approaches. Aim 2. To determine if intermolecular capsid protein interactions within the nucleocapsid undergo large-scale rearrangements during VEE virus maturation by determining the structure of pre-viral, viral, and post-viral nucleocapsids.