MR IMAGING OF MOUSE BRAIN AND CRANIOFACIAL BIRTH DEFECT MODEL

小鼠大脑和颅面先天缺陷模型的 MR 成像

• 批准号: 8363219
• 负责人: KATHLEEN K SULIK
• 金额: $ 1.84万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363219
• 关键词: Affect; Brain; Congenital Abnormality; Defect; Diffusion; Dose; Embryo; Ethanol; Exposure to; Face; Funding; Grant; Holoprosencephaly; Image; Laboratories; Magnetic Resonance Imaging; Microscopy; Modeling; Mus; National Center for Research Resources; Phenotype; Predisposition; Principal Investigator; Protocols documentation; Research; Research Infrastructure; Resources; Shapes; Source; Specimen; Staging; Surface; United States National Institutes of Health; Weight; Work; brain malformation; brain morphology; cost; craniofacial; fetal; smoothened signaling pathway

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Holoprosencephaly-spectrum defects can be induced by embryonic exposure to ethanol or Hedgehog signaling antagonists. Our first aim seeks to define the dose- and stage-dependent effects of Hedgehog signaling antagonist exposure through detailed characterization of CNS and craniofacial phenotype in affected fetal specimens. Our second aim is to apply MR microscopy in the study of facial morphometrics. Face shape is known to be useful in predicting both underlying brain morphology as well as predicting predisposition for congenital malformations. We will use MR acquired 3D facial images to identify morphological features of the face that are associated with specific brain malformations and to determine whether facial clefting can be predicted by subtle (non-clefting) morphometric changes. We intend for the first aim described above to be inter-relatable with work already performed in the Sulik laboratory defining the craniofacial and CNS phenotype of fetal mice exposed to ethanol embryonically. Thus, we will use the existing MRM protocol. We anticipate that the second research aim, utilizing MR for facial morphometric analysis, will be best served by modifying this protocol used for Aim1 by the addition of diffusion weighting to optimize the image quality of the facial surface.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 胚胎暴露于乙醇或Hedgehog信号拮抗剂可导致全前脑频谱缺陷。我们的第一个目标是通过详细描述受影响的胎儿标本的中枢神经系统和颅面表型，确定Hedgehog信号拮抗剂暴露的剂量和阶段依赖效应。我们的第二个目标是将磁共振显微镜应用于面部形态测量学的研究。众所周知，脸型在预测潜在的大脑形态以及预测先天畸形的易感性方面都是有用的。我们将使用MR获得的3D面部图像来识别与特定大脑畸形相关的面部形态特征，并确定是否可以通过细微的(非裂隙)形态测量变化来预测面部裂隙。 我们打算将上面描述的第一个目的与Sulik实验室已经进行的工作相互关联，这些工作定义了胚胎暴露于乙醇的胎鼠的颅面和中枢神经系统表型。因此，我们将使用现有的MRM协议。 我们预计，利用MR进行面部形态测量分析的第二个研究目标，将通过添加扩散加权来优化面部表面的图像质量来修改用于Aim1的协议，从而最好地满足第二个研究目标。