OXIDATIVE POST-TRANSLATIONAL MODIFICATIONS IN CARDIOVASCULAR DISEASE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在其资助的第一个7年期间，NHLBI赞助的血管蛋白质组学中心的研究重点是探索与心血管疾病相关的氧化翻译后修饰。核心实验室开发了适当的方法，并提供了二维凝胶分离或多级HPLC纯化蛋白质样品中肽的capLC-QTOF分析。结合资源，还进行了MALDI-TOF MS、LTQ-Orbitrap MS/MS和FTMS质谱分析。该中心制作了协助数据处理和解释的软件。一种新型的VC-MALDI低温傅里叶变换质谱仪与14-T磁铁的构建，使高通量，高性能分析的蛋白质水解混合物的生物来源，并有效，准确地确定其翻译后修饰。基础和临床研究人员，主要位于BUSM和HMS，正在使用蛋白质组学方法来研究氧化应激对细胞培养物，组织和整个生物体的影响。方案协调会的第一个供资期延长到本报告年度初，一些最后文件和会议发言也已完成。该项目为2010年8月开始的新项目奠定了基础，并为MS Resource开展的许多蛋白质组学研究奠定了基础。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the first 7-year period of its funding, the research of the NHLBI-sponsored Cardiovasculaar Proteomics Center focused on exploring the oxidative post-translational modifications that occur in conjunction with cardiovascular disease. The Core laboratory developed appropriate methods and provided capLC-QTOF analysis of peptides in digests of 2D-gel separated or multistage HPLC purified proteins. In conjunction with the Resource, MALDI-TOF MS, LTQ-Orbitrap MS/MS and FTMS mass spectral analyses were also carried out. Software to assist in data processing and interpretation was generated in the Center. A novel VC-MALDI cryogenic Fourier transform mass spectrometer with a 14-T magnet was constructed to enable high throughput, high performance analysis of proteolysis mixtures from biological sources, and efficient, accurate determination of their post-translational modifications. Basic and clinical investigators, primarily located at BUSM and HMS, are using proteomic approaches to investigate the effects of oxidative stress on cell cultures, tissues and whole organisms. The first period of the CPC funding extended into the beginning of this reporting year, and a number of the final papers and meeting presentations also were completed. This project laid the foundation for the new project that began in August 2010, and for many proteomics studies that have been undertaken by the MS Resource.