Legacy Support During Closure of the Mass Spectrometry Resource for Biology and Medicine

波士顿大学生物和医学质谱学资源的使命是 先进的、基于质谱学的方法的发展和应用 与人类健康和疾病相关的生物聚合物的特征。它的稳定性 基础科学家、临床医生之间密切的工作关系促进了进步 和实习生。由于NIGMS现在对P41赠款设定了时间限制，因此赠款P41 GM104603不能续订。这项提案要求提供必要的资金，以避免 资源活动的中断性突然关闭并提供平稳的过渡期 能够有序地完成正在进行的推动生物医学和协作的项目 依靠资源中唯一可用的方法和专业知识，最终确定正在进行的 软件开发，共享软件、数据库和协议，以满足 社区，以及确定和实施替代机制，以解决 用户社区的未来需求，并支持进一步的技术开发， 从该资源在过去20年中取得的成就演变而来。资源将 重点关注糖生物学对详细结构阐明的需求和对 多聚糖的复杂混合物。阐明糖链断裂途径的参考数据 使用多种解离方法将被汇编并存入公共数据库； 该资源正在为多糖分析创建的新的、用户友好的生物信息学工具将是 完成、测试和共享。离子移动性的协议和参考数据集.串联 将编制用于分析多糖和复杂多肽混合物的质谱图， 用于表征非共价络合物，并用于评估新的EXD电池设计 由一家小型行业合作者建造。内部开发的葡聚糖软件 变异修饰语和翻译后修饰语的结构确定和自上而下的分析 可以在各个蛋白质上的PTM之间建立联系的蛋白质将被完成 和共享；它应该为直接的临床分析和探测提供手段 蛋白质-多聚糖和蛋白质-蛋白质相互作用。待完成的翻译项目将 包括N-和O-连接多糖和脂多糖的特性 细菌和寄生虫糖脂和糖胺聚糖的结构测定 母乳、蛋白质错误折叠障碍、免疫学、细胞外基质生物学和 心血管疾病。该资源将转移其在培训学生方面的经验， 博士后研究员和实习科学家到其他教育机构和行业。

The mission of the Boston University Mass Spectrometry Resource for Biology and Medicine is the development and application of advanced, mass spectrometry-based methods for the characterization of biopolymers that are relevant to human health and disease. Its steady progress has been fostered by close working relationships between basic scientists, clinicians and trainees. Because NIGMS has now imposed a time limit for P41 grants, the grant P41 GM104603 cannot be renewed. This proposal requests the funding essential to avoid a disruptive sudden closedown of the Resource's activities and afford a smooth transition period to enable the orderly completion of ongoing driving biomedical and collaborative projects that depend on methods and expertise uniquely available in the Resource, finalizing of ongoing software development, sharing of software, databases and protocols to meet the needs of the community, and the identification and implementation of alternative mechanisms to address the future needs of the user community and enable further technology developments that should evolve from the accomplishments of the Resource over the last 20 years. The Resource will focus on the needs of glycobiology for detailed structural elucidations and the profiling of complex mixtures of glycans. Reference data that illuminates glycan fragmentation pathways using multiple dissociation methods will be compiled and deposited into public databases; the new, user-friendly bioinformatics tools that the Resource is creating for glycan analysis will be completed, tested and shared. Protocols and sets of reference data for Ion mobility-tandem mass spectrometry will be compiled for the analysis of glycans and complex peptide mixtures, for characterization of noncovalent complexes, and for evaluation of new ExD cell designs being constructed by a small industry collaborator. In-house in developed software for glycan structural determinations and top-down analysis of variant and post-translationally modified proteins, that can establish relationships among PTMs on individual proteins, will be completed and shared; it should provide means for straightforward clinical analyses, and for probing protein-glycan and protein-protein interactions. Translational projects to be completed will include characterization of N- and O-linked glycans and lipopolysaccharides on infectious bacteria and parasites, structural determinations of glycolipids and glycosaminoglycans in human milk, protein misfolding disorders, immunology, extracellular matrix biology and cardiovascular disease. The Resource will transfer its experience in training students, postdoctoral fellows and practicing scientists to other educational institutions and industry.