Legacy Support During Closure of the Mass Spectrometry Resource for Biology and Medicine

生物学和医学质谱资源关闭期间的遗留支持

• 批准号: 10204050
• 负责人: Catherine E. Costello
• 金额: $ 53.99万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10204050
• 关键词: Address; Area; Automobile Driving; Bacteria; Biology; Biopolymers; Boston; Cardiovascular Diseases; Cells; Clinical; Communities; Complex; Complex Mixtures; Computer software; Data; Databases; Deposition; Development; Disease; Dissociation; Electrons; Evaluation; Extracellular Matrix; Fostering; Funding; Funding Agency; Future; Glycobiology; Glycolipids; Glycosaminoglycans; Grant; Health; Human; Human Milk; Immunology; Individual; Industry; Industry Collaboration; Infection; Institution; Link; Lipopolysaccharides; Mass Spectrum Analysis; Medicine; Methods; Mission; Monoclonal Antibody R24; National Institute of General Medical Sciences; Parasites; Pathway interactions; Peptides; Policies; Polysaccharides; Postdoctoral Fellow; Proteins; Protocols documentation; Quality Control; Request for Proposals; Research; Resources; Scientist; Structure; Testing; Time; Training; Universities; Variant; base; bioinformatics tool; design; experience; ion mobility; mass spectrometer; protein misfolding; protein protein interaction; recruit; software development; student training; tandem mass spectrometry; technology development; user-friendly

The mission of the Boston University Mass Spectrometry Resource for Biology and Medicine is the development and application of advanced, mass spectrometry-based methods for the characterization of biopolymers that are relevant to human health and disease. Its steady progress has been fostered by close working relationships between basic scientists, clinicians and trainees. Because NIGMS has now imposed a time limit for P41 grants, the grant P41 GM104603 cannot be renewed. This proposal requests the funding essential to avoid a disruptive sudden closedown of the Resource's activities and afford a smooth transition period to enable the orderly completion of ongoing driving biomedical and collaborative projects that depend on methods and expertise uniquely available in the Resource, finalizing of ongoing software development, sharing of software, databases and protocols to meet the needs of the community, and the identification and implementation of alternative mechanisms to address the future needs of the user community and enable further technology developments that should evolve from the accomplishments of the Resource over the last 20 years. The Resource will focus on the needs of glycobiology for detailed structural elucidations and the profiling of complex mixtures of glycans. Reference data that illuminates glycan fragmentation pathways using multiple dissociation methods will be compiled and deposited into public databases; the new, user-friendly bioinformatics tools that the Resource is creating for glycan analysis will be completed, tested and shared. Protocols and sets of reference data for Ion mobility-tandem mass spectrometry will be compiled for the analysis of glycans and complex peptide mixtures, for characterization of noncovalent complexes, and for evaluation of new ExD cell designs being constructed by a small industry collaborator. In-house in developed software for glycan structural determinations and top-down analysis of variant and post-translationally modified proteins, that can establish relationships among PTMs on individual proteins, will be completed and shared; it should provide means for straightforward clinical analyses, and for probing protein-glycan and protein-protein interactions. Translational projects to be completed will include characterization of N- and O-linked glycans and lipopolysaccharides on infectious bacteria and parasites, structural determinations of glycolipids and glycosaminoglycans in human milk, protein misfolding disorders, immunology, extracellular matrix biology and cardiovascular disease. The Resource will transfer its experience in training students, postdoctoral fellows and practicing scientists to other educational institutions and industry.

波士顿大学生物学和医学质谱资源的使命是 基于质谱的高级方法的开发和应用 与人类健康和疾病有关的生物聚合物的表征。它稳定 基础科学家，临床医生之间的紧密工作关系促进了进步 和受训者。由于Nigms现在对P41赠款施加了时间限制，因此赠款P41 GM104603无法续签。该提案要求资金避免 破坏性突然关闭了资源的活动，并提供了平稳的过渡期 为了使正在进行的驾驶生物医学和协作项目有序地完成这些项目 取决于资源中独特可用的方法和专业知识，最终确定 软件开发，共享软件，数据库和协议，以满足 社区，以及识别和实施替代机制，以解决 用户社区的未来需求，并实现应有的技术发展 从过去20年中资源的成就发展。资源将 专注于糖生物学的需求，以详细的结构阐明和分析 聚糖的复杂混合物。阐明聚糖碎片途径的参考文献 使用多种解离方法将被编译并存放到公共数据库中；这 资源为聚糖分析创建的新型，用户友好的生物信息学工具将是 完成，测试和共享。离子迁移率串联的协议和参考数据集 质谱法将进行编译，以分析聚糖和复杂的肽混合物， 用于表征非共价复合物，并评估新的EXD细胞设计 由小型行业合作者建造。在开发软件中的内部软件 变体和翻译后修改的结构确定和自上而下的分析 可以在单个蛋白质上建立PTM之间关系的蛋白质将完成 并共享；它应提供直接临床分析的手段，并进行探测 蛋白质聚糖和蛋白质 - 蛋白质相互作用。要完成的翻译项目将 包括在感染性的N-和O连接的聚糖和脂多糖的表征 细菌和寄生虫，糖脂的结构测定和糖胺聚糖在 人乳，​​蛋白质错误折叠障碍，免疫学，细胞外基质生物学和 心血管疾病。资源将转移其在培训学生中的经验， 博士后研究员和科学家向其他教育机构和工业界实践。

项目成果

Dissecting the Mechanism of the Nonheme Iron Endoperoxidase FtmOx1 Using Substrate Analogues.

• DOI: 10.1021/jacsau.2c00248
• 发表时间: 2022-07-25
• 期刊: JACS AU
• 影响因子: 8
• 作者: Zhu, Guoliang;Yan, Wupeng;Wang, Xinye;Cheng, Ronghai;Naowarojna, Nathchar;Wang, Kun;Wang, Jun;Song, Heng;Wang, Yuyang;Liu, Hairong;Xia, Xuekui;Costello, Catherine E;Liu, Xueting;Zhang, Lixin;Liu, Pinghua
• 通讯作者: Liu, Pinghua