CRYSTAL STRUCTURES OF N-TERMINAL ECTODOMAINS OF CADHERIN-23

CADHERIN-23 N 端胞外域的晶体结构

• 批准号: 8361628
• 负责人: RACHELLE GAUDET
• 金额: $ 0.68万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361628
• 关键词: Adhesions; Architecture; Cadherins; Calcium ion; Elasticity; Funding; Grant; Hair Cells; Inherited; Labyrinth; Link; Mediating; Molecular; Mutation; N-terminal; National Center for Research Resources; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Source; Structure; United States National Institutes of Health; cost; deafness; human CDH23 protein; mutant; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The hair-cell tip link, an essential component of the mechanotransduction apparatus in the inner ear, has been proposed to be formed partly by cadherin- 23 (CDH23) which is involved in hereditary deafness and belongs to the cadherin superfamily of proteins. Calcium ions are required for cadherin- mediated adhesion and also for maintaining the integrity of the tip link. Hair cells have an elastic gating spring, but it is unclear whether tip links have the appropriate elasticity. Certain mutations in CDH23 cause deafness, but it is not known how. In order to investigate the molecular architecture and elasticity of the tip link and its deafness related mutants we determined the x-ray crystallographic structures of key CDH23 domains involved on tip link assembly and deafness.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 已经提出，内耳机械转导设备的重要组成部分是内耳中的一项重要组成部分。钙离子是钙粘蛋白介导的粘附所必需的，也需要维持尖端链接的完整性。毛细胞具有弹性的弹簧，但尚不清楚尖端链接是否具有适当的弹性。 CDH23中的某些突变会引起耳聋，但尚不清楚。为了研究尖端链接的分​​子结构和弹性及其与耳聋相关的突变体，我们确定了尖端链接组装和耳聋涉及的关键CDH23域的X射线晶体学结构。