Research Support Core: Synthesis Core

研究支持核心：综合核心

• 批准号: 8249994
• 负责人: LARRY W ROBERTSON
• 金额: $ 20.99万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8249994
• 关键词: Acetylcysteine; Air; Antigens; Chicago; Collaborations; Diazomethane; Glutathione; In Vitro; Individual; Inorganic Sulfates; Maintenance; Polychlorinated Biphenyls; Preparation; Quality Control; Quinones; Research; Research Project Grants; Research Support; Source; Toxic effect; Universities; Unspecified or Sulfate Ion Sulfates; Work; aroclor 1242; experience; in vivo; interest; programs; repository

The Synthesis Core directly supports the research function of the University of lowa SBRP research program (isbrp) by providing PCB exposure mixtures as well as individual PCB congeners and PCB metabolites. Specific Aim 1 is the maintenance of PCB mixtures and the preparation/maintenance of analytical standards. The Synthesis Core will serve as a repository for technical PCB mixtures (e.g., Aroclors 1242 and 1254) and a synthetic PCB mixture that approximates the average PCB profile found in Chicago air. In addition, the Synthesis Core will maintain analytical standards that are developed in close collaboration with the Analytical Core from pure PCB/PCB metabolites. Specific Aim 2 is the synthesis of PCB congeners for use with the in vitro and in vivo studies of Research Projects #1, #2 and # 5. These PCB congeners will also be available to the Analytical Core for the preparation of analytical and quality control/assurance standards. Specific Aim 3 is the synthesis of methoxylated and hydroxylated PCB metabolites. Hydroxylated PCB metabolites will be used by Projects #1, #2, #3, and #5 for in vitro and in vivo studies. Both the hydroxylated and methoxylated PCB derivatives will also be provided to the Analytical Core as analytical standards for the identification and quantification of (unknown) metabolites. Specific Aim 4 encompasses the synthesis of miscellaneous compounds for the isbrp, including PCB quinones (Projects #1, #2 and #3), PCB glutathione/N-acetyl cysteine conjugates (Project #1), PCB immunogens (Project #1) and PCB sulfates (Project #3). The Synthesis Core will also provide diazomethane as derivatization agent as part of this Aim. This effort indirectly supports Projects #1, #2, #4, #5 and #6. All compounds will be synthesized, if necessary in large (gram) quantities, with straightforward synthetic approaches that are well established at the University of lowa. Drawing on their extensive experience with the synthesis of organohalogen compounds. Synthesis Core staff will be able to synthesize every compound of interest to the individual Research Projects.

通过提供PCB暴露混合物以及单个PCB同系物和PCB代谢物，合成核心直接支持爱荷华大学SBRP研究计划（isbrp）的研究功能。具体目标1是PCB混合物的维护和分析标准的制备/维护。合成核心将作为技术多氯联苯混合物（例如，Aroclors 1242和1254）和合成多氯联苯混合物的存储库，该混合物接近芝加哥空气中发现的平均多氯联苯概况。此外，合成核心将维护与分析中心密切合作开发的分析标准