DYNAMIC STRUCTURE OF L-O AND L-D PHASES IN SPM-DOPC-CHOLESTEROL BY ESR

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 为了将SPM/DOPC/胆固醇模型脂质系统的应用扩展到真实的质膜，通过ESR谱对相边界上的Lo和Ld相进行了动态表征，以与真实的质膜进行比较。 序参量和动态参量，如旋转扩散系数，可以通过用动态模型拟合ESR谱来获得，并且这些参量可以用于表征脂质相。 如果来自模型膜相的参数表现出与从真实的质膜中的相同自旋探针获得的那些参数相似的特性，则我们可以有把握地说，模型膜与真实的膜相似。 可以采用许多不同的膜自旋探针来增加表征的特异性以及增加对相态的理解。 这种表征结合“全球”的联络线配置的Lo+Ld相共存区域将允许一个更详细的模型脂筏。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. To expand the application of the SPM/DOPC/cholesterol model lipid system to real plasma membranes, the dynamic characterization of the Lo and Ld phases on the phase boundary by ESR spectra has been undertaken for comparison to real plasma membranes. The order parameter and dynamic parameters, such as rotational diffusion coefficients, can be obtained from fitting an ESR spectrum with a dynamic model, and these parameters can be used to characterize a lipid phase. If parameter from a model membrane phase exhibits similar characteristics to those parameters obtained from the same spin-probe in real plasma membranes, then we can say with confidence that the model membrane is similar to the real membrane. Many different membrane spin-probes can be employed to increase the specificity of the characterization as well as increasing the understanding of the phase state. This characterization combined with the "global" tie-line configuration for the Lo+Ld phase coexistence region will allow a more detailed model for lipid rafts.