PROTEOMICS WITH NANOSPRAY SHEATH FLOW CE-MS INTERFACE AND FAST SCANNING MS

使用 Nanospray Sheath Flow CE-MS 接口和快速扫描 MS 进行蛋白质组学

• 批准号: 8365823
• 负责人: Michael MacCoss
• 金额: $ 0.95万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365823
• 关键词: Anus; Biology; Coupling; Funding; Fungal Genome; Grant; National Center for Research Resources; Principal Investigator; Proteomics; Research; Research Infrastructure; Resources; Scanning; Source; United States National Institutes of Health; cost; instrument; instrumentation; research study; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Earlier in the decade, the limiting factor in CE-MS proteomic experiments were slow scanning instruments not catching up with fast and efficient CE separation. We revisited the concept of a proof-of principle MUDPIT experiment, performed by Tong and Yates at the turn of the century (Anal. Chem. 1999, 71, 2270-2278). We performed experiments on LTQ and simplified nanospray sheath flow CE-MS interface to see if it is possible to take advantage of fast CE separations as a competitive tool for proteomic experiments by coupling them with modern, fast scanning MS instrumentation.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在这十年的早期，CE-MS蛋白质组学实验的限制因素是慢扫描仪器无法赶上快速有效的CE分离。 我们重新审视了由Tong和Yates在世纪之交（Anal. 1999，71，2270-2278）。我们在LTQ和简化的nanospray鞘流CE-MS接口上进行了实验，以了解是否可以利用快速CE分离作为蛋白质组学实验的竞争工具，将其与现代快速扫描MS仪器相结合。