THE MECHANISM OF DPP TRANSPORTATION

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在苍蝇翼想象盘中，DPP梯度形成于局部来源，其中DPP是由毗邻前(A)-后(P)间隔边界的一条前细胞分泌的。翅盘由腔和两层细胞、中膜上皮和柱状细胞层组成。腔内的自由扩散不能产生梯度，因为DPP的分泌转运域已经与GFP融合，而融合的GFP未能形成梯度(Entchev等人，2000)。几种转运机制被提出，包括限制扩散、平面跨细胞、细胞质和精氨酸体。我们将通过用PhotoActietable-GFP标记DPP来解决这个问题，并使用FCS或单粒子跟踪等技术记录DPP-PAGFP脉冲的运动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. In the fly wing imaginal disc, a Dpp gradient forms from a localized source, where Dpp is secreted from a stripe of anterior cells that is adjacent to the anterior (A)-posterior (P) compartment boundary. The wing disc consists of a lumen and two layers of cells, a peripodial epithelium and a columnar cell layer. Free diffusion in the lumen can not produce the gradient because the secretory transport domains of Dpp have been fused to GFP, and that fusion GFP fails to form a gradient (Entchev et al., 2000). Several transport mechanisms are proposed involving restricted diffusion, planar transcytosis, cytonemes and argosomes. We are going to address this question by tagging Dpp with PhotoActivetable-GFP, and record the movement of a pulse of Dpp-PAGFP with techniques like FCS or single-particle tracking.