DNA SEQUENCING

DNA测序

• 批准号: 8468769
• 负责人: Brinda K Rana
• 金额: $ 13.17万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8468769
• 关键词: Accounting; Cancer Center; Cancer Center Support Grant; Complement; Computer software; Consultations; DNA; DNA Sequence; DNA Sequencing Shared Resource; Data; Funding; Genetic; Genome; Hour; Microsatellite Repeats; Names; Reading; Research; Research Personnel; Resource Sharing; Resources; Running; Services; Site; Time; base; cost effective; design; member; web site

Begun in 1996 and initially funded by the Director's discretionary funds, the DNA Sequencing Shared Resource provides fast, on-site sequencing services to Moores UCSD Cancer Center Members. Eighty-six per cent of the utilization of this Shared Resource is from Cancer Center members while CCSG support for this Resource accounts for 30% of the Resource's annual operating expenses. Support of this Resource by the CCSG provides very cost effective DNA sequencing services to Cancer Center Members. The Resouce has developed a campus-wide reputation for providing consistently high quality, fast, long-read (1000+) and inexpensive DNA sequencing services. SNP analyses and microsatellite sizing for genome research or other applications is provided through the use of GeneScan¿, Genotyper¿ and GeneMapper¿ software used with our two Applied Biosystems 3130xl Genetic Analyzers. The speedy, convenient service established by this Resource for its users is complemented by the ready availability of expert consultation on how to solve difficult sequencing problems, relating to particular runs of bases in some parts of the genome. Such advice and help is a special value-added feature this facility provides for Center investigators, which is not easily obtained commercially. Our services have been designed to maximize efficiency for the investigator. Investigators use our web site to register their orders and define their template names. The Resource provides a daily courier service to pick up in each lab primers and templates ready for sequencing. Sequencing data is returned electronically within a turnaround time of 24 to 48 hours.

DNA测序共享资源始于1996年，最初由主任的自由支配资金资助，为摩尔UCSD癌症中心成员提供快速，现场测序服务。86%的这一共享资源的利用来自癌症中心成员，而CCSG对这一资源的支持占资源年度业务费用的30%。CCSG对这一资源的支持为癌症中心成员提供了非常具有成本效益的DNA测序服务。 该资源已开发了一个校园范围内的声誉，提供一贯的高品质，快速，长读（1000+）和廉价的DNA测序服务。通过使用我们的两台Applied Biosystems 3130 xl基因分析仪使用的GeneScan <$、Genotyper <$和GeneMapper <$软件，可以进行基因组研究或其他应用的SNP分析和微卫星大小测定。 本资源为用户提供的快速、方便的服务得到了专家咨询的补充，专家咨询可以帮助解决与基因组某些部分的特定碱基运行有关的测序难题。这种建议和帮助是该设施为中心调查人员提供的一种特殊的增值功能，这在商业上是不容易获得的。 我们的服务旨在最大限度地提高调查人员的效率。调查人员使用我们的网站注册他们的订单并定义他们的模板名称。该资源提供每日快递服务，在每个实验室提取准备测序的引物和模板。测序数据在24至48小时的周转时间内以电子方式返回。