DNA SEQUENCING

DNA测序共享资源始于1996年，最初由主任的可自由支配资金资助，为摩尔加州大学圣迭戈分校癌症中心的成员提供快速的现场测序服务。这一共享资源的86%的使用来自癌症中心成员，而CCSG对该资源的支持占该资源年度运营费用的30%。CCSG对这一资源的支持为癌症中心成员提供了非常经济高效的DNA测序服务。 该资源已经在全校范围内建立了声誉，提供始终如一的高质量、快速、长期阅读(1000+)和廉价的DNA测序服务。通过使用GeneScan、Genotyper和GeneMapper软件，为基因组研究或其他应用程序提供SNP分析和微卫星大小调整，这些软件与我们的两台应用生物系统3130xl遗传分析仪一起使用。 这一资源为其用户建立的快速、方便的服务得到了随时可用的专家咨询的补充，这些专家咨询如何解决与基因组某些部分的特定碱基运行有关的困难测序问题。这样的建议和帮助是这个设施为中心调查人员提供的一个特殊的增值功能，这是很难从商业上获得的。 我们的服务旨在最大限度地提高调查员的效率。调查人员使用我们的网站注册他们的订单并定义他们的模板名称。该资源每天提供快递服务，以便在每个实验室领取准备好测序的引物和模板。测序数据在24至48小时的周转时间内以电子方式返回。

Begun in 1996 and initially funded by the Director's discretionary funds, the DNA Sequencing Shared Resource provides fast, on-site sequencing services to Moores UCSD Cancer Center Members. Eighty-six per cent of the utilization of this Shared Resource is from Cancer Center members while CCSG support for this Resource accounts for 30% of the Resource's annual operating expenses. Support of this Resource by the CCSG provides very cost effective DNA sequencing services to Cancer Center Members. The Resouce has developed a campus-wide reputation for providing consistently high quality, fast, long-read (1000+) and inexpensive DNA sequencing services. SNP analyses and microsatellite sizing for genome research or other applications is provided through the use of GeneScan¿, Genotyper¿ and GeneMapper¿ software used with our two Applied Biosystems 3130xl Genetic Analyzers. The speedy, convenient service established by this Resource for its users is complemented by the ready availability of expert consultation on how to solve difficult sequencing problems, relating to particular runs of bases in some parts of the genome. Such advice and help is a special value-added feature this facility provides for Center investigators, which is not easily obtained commercially. Our services have been designed to maximize efficiency for the investigator. Investigators use our web site to register their orders and define their template names. The Resource provides a daily courier service to pick up in each lab primers and templates ready for sequencing. Sequencing data is returned electronically within a turnaround time of 24 to 48 hours.