Core C: Imaging: from Super-Resolution to Whole Animal

项目概要-核心C Core C支持该计划中的每个项目， 技术和组织病理学服务，使计划研究人员能够使用成像来深入了解 分子和细胞机制，并跟踪肿瘤进展、治疗反应和转移。 细胞和动物的成像-从分子到组织，再到全身水平-都得到了Core的支持。 Core C将协助对固定细胞和组织进行亚细胞到全组织水平的分析， 通过与世界一流病理学家的合作提供组织病理学服务。Core还支持Live 三维和四维细胞成像（3D和4D，也称为3D延时），以及活体成像 动物Core C提供的成像方法包括几种尖端成像技术， 其中在世界范围内的有限数量的地点提供。这些包括i）超分辨率（100 nm）， 和用OMX（光学显微镜实验）显微镜实现的高速细胞成像; ii）3D 使用光片显微术的肿瘤和转移的全组织重建;和iii）活体（活的 动物）成像。 在即将到来的请求支持期间，核心C建议通过以下方式支持项目调查人员 四个目标。首先，该计划将提供组织学，免疫标记和组织病理学的帮助。 其次，Core将支持活细胞成像和超分辨率成像。第三，核心将协助 在组织水平成像，使用3D组织重建或动物活体成像。这些技术 每一种都需要通常不可用的定制显微镜。四、提供方案 研究人员使用工具跟踪肿瘤生长和转移，核心C将协助整个动物成像 方法，包括生物发光，荧光蛋白和小动物超声。核心C也将 协助使用高级成像软件，包括Image J（NIH）、Imaris（Bitplane）和Volocity （Perkin-Elmer）.新的更新是支持定量微转移，活体成像，通过 手术植入的窗口，以及使用光片显微镜的3D组织重建。 Core C拥有前所未有的成像平台，涵盖超分辨率成像、3D组织成像、 重建、组织中的活细胞跟踪和整个动物成像。一个核心，使程序 调查人员获得这些技术是必不可少的，因为大多数成像技术支持的 核心是高度复杂的，需要非常专业的训练。此外，获得文书 如果没有核心的支持，这些成像技术所需的成本将是高昂的。核心C 将为计划成员提供关键工具，并通过开发新的成像方法， 最终是更广泛的癌症研究界。

PROJECT SUMMARY – CORE C Core C, which supports every project within the Program, provides access to sophisticated imaging technologies and histopathology services, enabling Program investigators to use imaging to obtain insights into molecular and cellular mechanisms and to track tumor progression, therapy response, and metastasis. Imaging in cells and animals – from the molecular, to tissue, to whole-body level – is supported by the Core. Core C will assist with sub-cellular to whole-tissue level analysis of fixed cells and tissues, providing histopathological services through a subcontract with a world-class pathologist. The Core also supports live cell imaging in three and four dimensions (3D and 4D, also known as 3D time-lapse), and imaging in live animals. The imaging approaches offered by Core C include several cutting-edge imaging technologies, each of which is available in a limited number of locations worldwide. These include i) super-resolution (100 nm), and high-speed cellular imaging achieved with an OMX (Optical Microscope eXperimental) microscope; ii) 3D whole-tissue reconstruction of tumors and metastases using light-sheet microscopy; and iii) intravital (live animal) imaging using custom-built spinning disk confocal and multi-photon microscopes. During the upcoming period of requested support, Core C proposes to support Program investigators through four aims. First, the program will provide assistance with histology, immune labeling, and histopathology. Second, the Core will support live cell imaging and super-resolution imaging. Third, the Core will assist with imaging at the tissue level, either using 3D tissue reconstruction or live imaging in animals. These technologies each require custom-built microscopes that are not generally available. Fourth, to provide Program investigators with tools to track tumor growth and metastasis, Core C will assist with whole animal imaging approaches, including bioluminescence, fluorescent proteins, and small animal ultrasound. Core C will also assist with the use of advanced imaging software, including Image J (NIH), Imaris (Bitplane), and Volocity (Perkin-Elmer). New in this renewal is support for quantification of micrometastasis, intravital imaging through surgically implanted windows, and 3D tissue reconstruction using light-sheet microscopy. Together, Core C has an unprecedented imaging platform, covering super-resolution imaging, 3D tissue reconstruction, live cell tracking in tissues and whole animal imaging. A Core that enables program investigators access to these technologies is essential as most of the imaging technologies supported by the Core are highly sophisticated and require very specialized training. Furthermore, access to the instruments required for these imaging technologies would be cost-prohibitive without the support of a Core. Thus, Core C will provide critical tools for Program members and, through the development of new imaging approaches, ultimately the broader cancer research community.