Core C: Imaging: from Super-Resolution to Whole Animal

项目摘要-核心C 核心C支持该计划内的每个项目，提供对复杂成像的访问 技术和组织病理学服务，使计划调查人员能够使用成像来获得对 分子和细胞机制，并跟踪肿瘤进展、治疗反应和转移。 在细胞和动物中的成像-从分子、组织到全身水平-都得到了Core的支持。 核心C将协助对固定细胞和组织进行亚细胞到整个组织水平的分析，提供 通过与世界级病理学家签订分包合同提供组织病理学服务。酷睿还支持LIVE 三维和四维(3D和4D，也称为3D时间推移)的细胞成像，以及实时成像 动物。Core C提供的成像方法包括几种尖端成像技术，每一种 其中，在全球有限的几个地点都可以买到。这些包括i)超分辨率(100 Nm)， 以及用OMX(光学显微镜实验)显微镜实现的高速细胞成像；ii)3D 使用光片显微镜进行肿瘤和转移的全组织重建；以及三)活体(活体) 动物)使用定制的旋转圆盘共焦和多光子显微镜进行成像。 在即将到来的请求支持期间，Core C建议通过以下方式支持计划调查人员 四个目标。首先，该计划将在组织学、免疫标记和组织病理学方面提供帮助。 其次，Core将支持活细胞成像和超分辨率成像。第三，核心将协助 在组织水平上成像，使用3D组织重建或在动物身上进行实时成像。这些技术 每一种都需要定制的显微镜，而这些显微镜并不是普遍可用的。第四，提供方案 研究人员拥有追踪肿瘤生长和转移的工具，Core C将协助进行整体动物成像 方法，包括生物发光、荧光蛋白和小动物超声波。核心C也将 协助使用高级成像软件，包括Image J(NIH)、Imaris(BitPlane)和Volocity (Perkin-Elmer)。这次更新的新功能是支持对微转移的量化，通过以下方式进行活体成像 通过外科手术植入的窗户，以及使用光片显微镜进行的3D组织重建。 总而言之，Core C拥有前所未有的成像平台，涵盖了超分辨率成像、3D组织 重建、组织中的活细胞跟踪和整个动物的成像。使程序成为可能的核心 调查人员获得这些技术是至关重要的，因为 核心是高度复杂的，需要非常专门的培训。此外，获得这些仪器的机会 如果没有核心的支持，这些成像技术所需的成本将是令人望而却步的。因此，核心C 将为计划成员提供关键工具，并通过开发新的成像方法， 归根结底，更广泛的癌症研究界。

PROJECT SUMMARY – CORE C Core C, which supports every project within the Program, provides access to sophisticated imaging technologies and histopathology services, enabling Program investigators to use imaging to obtain insights into molecular and cellular mechanisms and to track tumor progression, therapy response, and metastasis. Imaging in cells and animals – from the molecular, to tissue, to whole-body level – is supported by the Core. Core C will assist with sub-cellular to whole-tissue level analysis of fixed cells and tissues, providing histopathological services through a subcontract with a world-class pathologist. The Core also supports live cell imaging in three and four dimensions (3D and 4D, also known as 3D time-lapse), and imaging in live animals. The imaging approaches offered by Core C include several cutting-edge imaging technologies, each of which is available in a limited number of locations worldwide. These include i) super-resolution (100 nm), and high-speed cellular imaging achieved with an OMX (Optical Microscope eXperimental) microscope; ii) 3D whole-tissue reconstruction of tumors and metastases using light-sheet microscopy; and iii) intravital (live animal) imaging using custom-built spinning disk confocal and multi-photon microscopes. During the upcoming period of requested support, Core C proposes to support Program investigators through four aims. First, the program will provide assistance with histology, immune labeling, and histopathology. Second, the Core will support live cell imaging and super-resolution imaging. Third, the Core will assist with imaging at the tissue level, either using 3D tissue reconstruction or live imaging in animals. These technologies each require custom-built microscopes that are not generally available. Fourth, to provide Program investigators with tools to track tumor growth and metastasis, Core C will assist with whole animal imaging approaches, including bioluminescence, fluorescent proteins, and small animal ultrasound. Core C will also assist with the use of advanced imaging software, including Image J (NIH), Imaris (Bitplane), and Volocity (Perkin-Elmer). New in this renewal is support for quantification of micrometastasis, intravital imaging through surgically implanted windows, and 3D tissue reconstruction using light-sheet microscopy. Together, Core C has an unprecedented imaging platform, covering super-resolution imaging, 3D tissue reconstruction, live cell tracking in tissues and whole animal imaging. A Core that enables program investigators access to these technologies is essential as most of the imaging technologies supported by the Core are highly sophisticated and require very specialized training. Furthermore, access to the instruments required for these imaging technologies would be cost-prohibitive without the support of a Core. Thus, Core C will provide critical tools for Program members and, through the development of new imaging approaches, ultimately the broader cancer research community.