Core C

Core C提供的服务将使该计划项目能够以经济高效的方式将迫切需要的组织病理学服务和创新而强大的成像技术纳入该计划。Core C通过提供对复杂成像技术的访问，支持该计划中的每个项目。超分辨率成像、三维(3D)组织重建、动物活细胞成像和整体动物成像将在下一个申请资金的时期对该计划产生重大影响，实际上已经开始这样做了。Core C正在进行的创新将使这些技术成为用于癌症研究的强大生物工具。重要的是，Core C还通过与世界级病理学家的分包合同为该计划提供病理支持。在即将到来的请求支持期间，核心建议通过四个总体目标支持方案调查人员。首先，该计划将在组织学、免疫标记和病理学方面提供帮助。其次，Core将支持超分辨率成像和使用全球仅有的20台OMX显微镜中的一台进行活细胞成像。第三，核心将在组织水平上协助成像，无论是使用3D组织重建还是在动物身上进行实时成像。这些技术中的每一项都需要定制的显微镜，而这些显微镜并不常见。第四，为了向项目研究人员提供跟踪肿瘤生长和转移的工具，核心将协助整个动物成像方法，包括生物发光、荧光蛋白和小动物超声波。总而言之，Core拥有一个前所未有的成像平台，涵盖超分辨率成像、3D组织重建、组织中的活细胞跟踪和整体动物成像。使项目调查人员能够使用这些技术的核心是至关重要的，因为核心支持的大多数成像技术都非常复杂，需要非常专业的培训。此外，如果没有核心的支持，获取这些成像技术所需的仪器将是成本高昂的。

The services provided in Core C will enable this Program Project to incorporate highly needed histopathology services and innovative and powerful imaging technology into the program in a cost-effective manner. Core C supports every project within the program by providing access to sophisticated imaging technologies. Super-resolution imaging, 3-dimensional (3D) tissue reconstruction, live cell imaging in animals and whole animal imaging will strongly impact the Program during the next period of requested funding, and indeed has already begun to do so. Ongoing innovations by Core C will make these technologies powerful biological tools for the use in cancer research. Importantly, Core C also provides pathological support for the Program through a subcontract with a world-class pathologist. During the upcoming period of requested support, the core proposes to support Program investigators through four general aims. First, the program will provide assistance with histology, immune labeling and pathology. Second, the Core will support super-resolution imaging and live cell imaging using one of only about twenty OMX microscopes that exists worldwide. Third, the core will assist with imaging at the tissue level, either using 3D tissue reconstruction or live imaging in animals. These technologies each require custom built microscopes that are not generally available. Fourth, to provide Program investigators with tools to track tumor growth and metastasis, the Core will assist with whole animal imaging approaches, including bioluminescence, fluorescent proteins and small animal ultrasound. Together, the Core have an unprecedented imaging platform, covering super-resolution imaging, 3D tissue reconstruction, live cell tracking in tissues and whole animal imaging. A core that enables program investigators access to these technologies is essential as most of the imaging technologies supported by the Core are highly sophisticated and require very specialized training. Furthermore, access to the instruments required for these imaging technologies would be cost-prohibitive without the support of a core.