Core C: Animal Modeling and Imaging

核心C-动物建模和成像 项目总结 Core C支持该计划内的所有项目，为以有机化合物为基础的复杂动物提供通道 模型、小动物成像技术、组织病理学以及器官和肿瘤样本的成像。这些 服务使项目调查人员能够深入了解导致肿瘤的分子和细胞机制 进展、治疗反应和转移。核心C将协助设计动物实验和 支持活体动物成像，从经典的生物发光成像(BLI)到最先进的成像 技术，仅在世界范围内有限数量的地点可用，如小动物正电子发射 断层扫描(PET)和单光子发射计算机断层扫描(SPECT)。核心还将支持 使用定制的记者对不同的细胞信号通路进行成像。组织病理学服务将是 通过与一位专门研究老鼠癌症模型的世界级病理学家签订的顾问协议提供。 最后，核心将支持固定有机物和肿瘤组织的免疫标记，包括支持整个- 安装三维(3D)体积扫描与抗体标记和多重免疫组织化学。 在即将到来的请求支持期间，Core C建议通过以下方式支持计划调查人员 三个目标。首先，该计划将为使用癌细胞移植的动物建模提供帮助 细胞系和有机体来源的癌细胞。基于有机化合物的动物建模需要对每一行进行优化，以 在注射前实现细胞的最佳解离。第二，核心将为项目调查人员提供 跟踪肿瘤生长、转移和治疗反应的工具，通过辅助小动物BLI、超声波、 PET、SPECT和计算机断层扫描(CT)成像。使用正电子发射计算机断层扫描，SPECT， 而CT不仅需要复杂的成像设备和记者，还需要相当多的专业知识。第三, 该核心将协助动物组织的分析，从组织病理学评估到标准 免疫标记、多重免疫组织化学和最新的抗体三维体积重建 贴标签。最后，Core C将协助使用先进的成像软件，包括Image J(NIH)和 Imaris(比特机)。此次更新的新功能是支持活体动物和多路传输中的CT、PET和SPECT成像 固定器官和组织标本的免疫组织化学和三维体积重建。 总之，Core C为动物建模和成像提供了一个全面的平台，涵盖了所有必要的 表征体内肿瘤生长的方法。一个核心，使程序调查人员能够访问这些 技术是必不可少的，因为核心支持的大多数服务都需要专业知识和 训练。因此，核心C将为计划成员提供关键工具，并通过开发新的 成像方法，归根结底是更广泛的癌症研究社区。

CORE C - ANIMAL MODELING AND IMAGING PROJECT SUMMARY Core C supports all projects within the Program by providing access to sophisticated organoid-based animal models, small-animal imaging technologies, histopathology, and imaging of organoid and tumor samples. These services enable Program investigators to obtain insights into molecular and cellular mechanisms driving tumor progression, therapy response, and metastasis. Core C will assist with the design of animal experiments and support live animal imaging from classical bioluminescence imaging (BLI) to state-of-the-art imaging technologies, available only in a limited number of locations worldwide, such as small-animal positron emission tomography (PET) and single-photon emission computed tomography (SPECT). The Core will also support imaging with customized reporters for different cell signaling pathways. Histopathological services will be provided through a consultant agreement with a world-class pathologist specializing in mouse models of cancer. Finally, the Core will support immunolabeling of fixed organoids and tumor tissues, including support for whole- mount 3-dimensional (3D) volume scanning with antibody labeling and for multiplex immunohistochemistry. During the upcoming period of requested support, Core C proposes to support Program investigators through three aims. First, the program will provide assistance with animal modeling using transplantation with cancer cell lines and organoid-derived cancer cells. Organoid-based animal modeling requires optimization for each line to achieve optimal dissociation of cells before injection. Second, the core will provide Program investigators with tools to track tumor growth, metastasis, and therapy responses by assisting with small-animal BLI, ultrasound, PET, SPECT, and computed tomography (CT) imaging. Conducting imaging experiments with PET, SPECT, and CT requires not only sophisticated imaging equipment and reporters but also considerable expertise. Third, the core will assist with the analysis of tissues from animals, from histopathological evaluations to standard immune labeling, multiplex immunohistochemistry, and state-of-the-art 3D volume reconstruction with antibody labeling. Finally, Core C will assist with the use of advanced imaging software, including Image J (NIH) and Imaris (Bitplane). New in this renewal is support for CT, PET, and SPECT imaging in live animals and multiplex immunohistochemistry and 3D volume reconstruction on fixed organoid and tissue samples. Together, Core C provides a comprehensive platform for animal modeling and imaging, covering all necessary approaches for characterizing tumor growth in vivo. A Core that enables program investigators access to these technologies is essential as most of the services supported by the Core require specialized expertise and training. Thus, Core C will provide critical tools for Program members and, through the development of new imaging approaches, ultimately the broader cancer research community.