Core C: Animal Modeling and Imaging

核心 C：动物建模和成像

• 批准号: 10555937
• 负责人: Mikala Egeblad
• 金额: $ 21.5万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-10 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10555937
• 关键词: 3-Dimensional; Address; Affect; Agreement; Animal Cancer Model; Animal Experiments; Animal Model; Animals; Antibodies; Antisense Oligonucleotides; Automobile Driving; Biological Models; Cancer Model; Cancer cell line; Cells; Collaborations; Collection; Communities; Data; Data Set; Development; Dissociation; Doctor of Philosophy; Employment; Engraftment; Ensure; Equipment; Evaluation; Funding; Genetic; Genetic Transcription; Goals; Harvest; Histopathology; Image; Imaging technology; Immune; Immunohistochemistry; In Situ Hybridization; Individual; Injections; Investigation; Kinetics; Label; Leadership; Location; Malignant Neoplasms; Mammary gland; Methods; Microscope; Modernization; Modification; Molecular; Mouse Strains; Nature; Neoplasm Metastasis; Organoids; Pancreas; Pathologic; Pathologist; Positron-Emission Tomography; RNA Splicing; Reporter; Research Personnel; Resolution; Sampling; Scanning; Services; Signal Pathway; Signal Transduction; Structure; System; Technology; Time; Tissue Sample; Tissues; Training; Transplantation; Tumor Burden; Tumor Tissue; United States National Institutes of Health; Work; X Inactivation; X-Ray Computed Tomography; animal imaging; animal model selection; animal tissue; anticancer research; bioluminescence imaging; cancer cell; cancer imaging; cohort; cost; cost effective; design; experience; experimental study; high resolution imaging; imaging approach; imaging modality; imaging software; imaging system; in vivo; in vivo imaging; innovation; innovative technologies; insight; malignant breast neoplasm; member; molecular imaging; molecular marker; mouse model; novel; novel imaging technology; pancreatic cancer model; pharmacologic; programs; reconstruction; single photon emission computed tomography; technology development; technology validation; tissue fixing; tool; transplant model; treatment response; tumor; tumor diagnosis; tumor growth; tumor heterogeneity; tumor progression; ultrasound

CORE C - ANIMAL MODELING AND IMAGING PROJECT SUMMARY Core C supports all projects within the Program by providing access to sophisticated organoid-based animal models, small-animal imaging technologies, histopathology, and imaging of organoid and tumor samples. These services enable Program investigators to obtain insights into molecular and cellular mechanisms driving tumor progression, therapy response, and metastasis. Core C will assist with the design of animal experiments and support live animal imaging from classical bioluminescence imaging (BLI) to state-of-the-art imaging technologies, available only in a limited number of locations worldwide, such as small-animal positron emission tomography (PET) and single-photon emission computed tomography (SPECT). The Core will also support imaging with customized reporters for different cell signaling pathways. Histopathological services will be provided through a consultant agreement with a world-class pathologist specializing in mouse models of cancer. Finally, the Core will support immunolabeling of fixed organoids and tumor tissues, including support for whole- mount 3-dimensional (3D) volume scanning with antibody labeling and for multiplex immunohistochemistry. During the upcoming period of requested support, Core C proposes to support Program investigators through three aims. First, the program will provide assistance with animal modeling using transplantation with cancer cell lines and organoid-derived cancer cells. Organoid-based animal modeling requires optimization for each line to achieve optimal dissociation of cells before injection. Second, the core will provide Program investigators with tools to track tumor growth, metastasis, and therapy responses by assisting with small-animal BLI, ultrasound, PET, SPECT, and computed tomography (CT) imaging. Conducting imaging experiments with PET, SPECT, and CT requires not only sophisticated imaging equipment and reporters but also considerable expertise. Third, the core will assist with the analysis of tissues from animals, from histopathological evaluations to standard immune labeling, multiplex immunohistochemistry, and state-of-the-art 3D volume reconstruction with antibody labeling. Finally, Core C will assist with the use of advanced imaging software, including Image J (NIH) and Imaris (Bitplane). New in this renewal is support for CT, PET, and SPECT imaging in live animals and multiplex immunohistochemistry and 3D volume reconstruction on fixed organoid and tissue samples. Together, Core C provides a comprehensive platform for animal modeling and imaging, covering all necessary approaches for characterizing tumor growth in vivo. A Core that enables program investigators access to these technologies is essential as most of the services supported by the Core require specialized expertise and training. Thus, Core C will provide critical tools for Program members and, through the development of new imaging approaches, ultimately the broader cancer research community.

核心C -动物建模和成像 项目摘要 核心C通过提供先进的类器官动物模型来支持该计划中的所有项目。 模型、小动物成像技术、组织病理学以及类器官和肿瘤样品的成像。这些 这些服务使项目研究人员能够深入了解驱动肿瘤的分子和细胞机制 进展、治疗反应和转移。核心C将协助设计动物实验， 支持从经典生物发光成像（BLI）到最先进成像活体动物成像 技术，仅在全世界有限的几个地点可用，例如小动物正电子发射 断层扫描（PET）和单光子发射计算机断层扫描（SPECT）。核心还将支持 使用定制的报告分子成像，用于不同的细胞信号传导途径。生物学服务将 通过与专门研究小鼠癌症模型的世界级病理学家的顾问协议提供。 最后，核心将支持固定的类器官和肿瘤组织的免疫标记，包括支持整个 用抗体标记进行三维（3D）体积扫描，并用于多重免疫组织化学。 在即将到来的请求支持期间，核心C建议通过以下方式支持项目调查人员 三个目标。首先，该计划将提供使用癌细胞移植的动物模型的援助 细胞系和类器官来源的癌细胞。基于类器官的动物建模需要对每条线进行优化， 在注射前实现细胞的最佳解离。第二，核心将为计划调查人员提供 通过辅助小动物BLI，超声， PET、SPECT和计算机断层扫描（CT）成像。用PET，SPECT， 而CT不仅需要精密的成像设备和记录仪，还需要相当多的专业知识。第三、 核心将有助于分析动物组织，从组织病理学评价到标准 免疫标记、多重免疫组织化学和最先进的抗体3D体积重建 标签。最后，Core C将协助使用先进的成像软件，包括Image J（NIH）和 Imaris（位平面）。此次更新的新功能是支持活体动物的CT、PET和SPECT成像， 免疫组织化学和3D体积重建。 Core C为动物建模和成像提供了一个全面的平台，涵盖了所有必要的 用于表征体内肿瘤生长的方法。使项目调查人员能够访问这些内容的核心 技术是必不可少的，因为核心支持的大多数服务都需要专业知识， 训练因此，核心C将为计划成员提供关键工具，并通过开发新的 成像方法，最终更广泛的癌症研究社区。