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Single Cell Measurement Core Facility

单细胞测量核心设施

基本信息

批准号：
8555278
负责人：
Gregoire Altan-Bonnet
金额：
$ 34.01万
依托单位：
DANA-FARBER CANCER INST
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-30 至 2014-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8555278
关键词：
Accounting Address Adenocarcinoma Cell Animals Antibodies Apoptotic Asphyxia Biological Assay Brain Neoplasms Calibration Carbon Dioxide Caring Cell Cycle Cell Death Cell Line Cell Proliferation Cell Survival Cells Chemicals Collaborations Collection Color Colorectal Communities Computer software Core Facility Custom Data Data Collection Databases Death Rate Development Differentiation Antigens Dislocations Dyes Epidermal Growth Factor Receptor Epithelial Cells Euthanasia Exposure to Flow Cytometry Fluorescence Freezing Frequencies Genotype Glioma Growth Hand Hematopoietic Hematopoietic Neoplasms Hemorrhage Hydrogen Peroxide Hypoxia IACUC Leukocytes Ligands Liquid substance Lung Adenocarcinoma Lung Neoplasms Lymphocyte MAP Kinase Gene Malignant Neoplasms Measurement Measures Memorial Sloan-Kettering Cancer Center Methanol Modeling Molecular Target Monitor Monoclonal Antibodies Mus Mutation Nitrogen Noise Pathway interactions Patients Peripheral Blood Mononuclear Cell Pharmaceutical Preparations Phase Phospho-Specific Antibodies Phosphoproteins Phosphoric Monoester Hydrolases Phosphotransferases Population Population Heterogeneity Preparation Primary Neoplasm Procedures Process Proteins Proto-Oncogene Proteins c-akt Protocols documentation Publications Quality Control Recovery Research Research Personnel Resolution Resources Rest Robotics Sampling Set protein Signal Transduction Small Interfering RNA Sodium Vanadate Staining method Stains Surface System Temperature Testing Time Validation Western Blotting Work cell type collagenase computerized data processing crosslink data sharing design digital experience flexibility glioma cell line leukemia mathematical model mouse model neoplastic cell new technology oncology phosphatase inhibitor physical science receptor research study response sample collection sample fixation tumor

项目摘要

The Research Resource Core A will perform quantitative single-cell assays and assist the investigators of the Physical Science - Oncology Center (PS-OC) to perform the research proposed in Projects 1, 2 and 3. This core facility addresses the need for new technologies that enable the monitoring ofthe functional response of primary tumor cells in their native context. To achieve this, we will rely on recent developments in flow cj/tometry that multiplex measurements of phenotypic variability, functional state of activation and/or the response to drugs in heterogeneous populations of cells. Specifically, this core will focus on measuring phospho-profiles of drugsensitive pathways (e.g. EGFR), downstream signaling cascades (e.g. AKT and MAPK pathways), cell-cycle phases, and apoptotic response at the single cell level. Special emphasis will be given to quantification of parameters for the djmamics of tumor cell proliferation and death (e.g. during drug treatment with varying concentration). This core will contribute to the PS-OC by providing quantitative measurements that parameterize

研究资源核心A将进行定量单细胞测定，并协助研究人员进行研究。物理科学-肿瘤学中心（PS-OC）执行项目1、2和3中提出的研究。这一核心设施解决了对新技术的需求，使监测功能反应的主要肿瘤细胞在它们的自然环境中。为了实现这一点，我们将依赖于流量计的最新发展，多重测量表型变异性、激活的功能状态和/或对药物的反应，异质细胞群。具体而言，该核心将专注于测量药物敏感性的磷酸化特征。途径（例如EGFR）、下游信号级联（例如AKT和MAPK途径）、细胞周期时相和单细胞水平的凋亡反应。将特别强调以下方面的量化：肿瘤细胞增殖和死亡的动态参数（例如，在药物治疗期间，浓度）。该核心将通过提供定量测量来促进PS-OC，参数化