Single Cell Measurement Core Facility

研究资源核心A将进行定量的单细胞分析，并协助 物理科学-肿瘤学中心(PS-OC)执行项目1、2和3中提出的研究。这一核心 设施满足了对新技术的需求，使其能够监测初级保健人员的功能反应 肿瘤细胞在它们的自然环境中。为了实现这一点，我们将依赖于Flow CJ/Tometer的最新发展 表型变异性、激活功能状态和/或对药物反应的多重测量 不同种类的细胞。具体地说，这个核心将专注于测量药物敏感的磷酸盐谱 通路(如EGFR)、下游信号级联通路(如AKT和MAPK通路)、细胞周期 在单细胞水平上的凋亡反应。将特别强调量化 肿瘤细胞增殖和死亡的参数(例如，在不同的药物治疗期间 浓度)。该核心将通过提供定量测量来为PS-OC做出贡献 参数化

The Research Resource Core A will perform quantitative single-cell assays and assist the investigators of the Physical Science - Oncology Center (PS-OC) to perform the research proposed in Projects 1, 2 and 3. This core facility addresses the need for new technologies that enable the monitoring ofthe functional response of primary tumor cells in their native context. To achieve this, we will rely on recent developments in flow cj/tometry that multiplex measurements of phenotypic variability, functional state of activation and/or the response to drugs in heterogeneous populations of cells. Specifically, this core will focus on measuring phospho-profiles of drugsensitive pathways (e.g. EGFR), downstream signaling cascades (e.g. AKT and MAPK pathways), cell-cycle phases, and apoptotic response at the single cell level. Special emphasis will be given to quantification of parameters for the djmamics of tumor cell proliferation and death (e.g. during drug treatment with varying concentration). This core will contribute to the PS-OC by providing quantitative measurements that parameterize