Dynamics of viral infection

β-冠状病毒是一个正链包膜RNA病毒家族，包括 严重急性呼吸综合征-CoV 2（SARS-CoV 2）。虽然很多人都知道， 细胞进入和复制途径，它们的出口模式仍然不确定;然而， 与其他包膜病毒类似，假定通过生物合成分泌途径。 使用成像方法结合病毒特异性报告基因，我们证明， β-冠状病毒利用溶酶体运输从细胞中排出。该途径 受Arf样小GT受体Arl 8b调节;因此，病毒排出对抑制剂不敏感 生物合成分泌途径。冠状病毒感染导致溶酶体 脱酸、溶酶体降解失活和抗原呈递破坏 途径。这种冠状病毒诱导的溶酶体利用提供了对 在患者中观察到细胞和免疫学异常，并提示新的治疗方法 方式。

Beta-Coronaviruses are a family of positive-strand enveloped RNA viruses that include the severe acute respiratory syndrome-CoV2 (SARS-CoV2). While much is known regarding their cellular entry and replication pathways, their mode of egress remains uncertain; however, this is assumed to be via the biosynthetic secretory pathway by analogy to other enveloped viruses. Using imaging methodologies in combination with virus-specific reporters, we demonstrated that beta-Coronaviruses utilize lysosomal trafficking for egress from cells. This pathway is regulated by the Arf-like small GTPase Arl8b; thus, virus egress is insensitive to inhibitors of the biosynthetic secretory pathway. Coronavirus infection results in lysosome deacidification, inactivation of lysosomal degradation and disruption of antigen presentation pathways. This coronavirus-induced exploitation of lysosomes provides insights into the cellular and immunological abnormalities observed in patients and suggests new therapeutic modalities.