Acute and Chronic In vivo Effects of Engineered Nanomaterials

本研究的重点是纳米粒子的物理化学特性在其通过吸入途径与体内暴露后与细胞、细胞器和器官相互作用中的作用。在第一年，我们将评估三种不同物理化学状态下三种选定纳米粒子的生物动力学和毒性。 我们研究的一个重要目标将是评估吸入纳米颗粒的急性毒性和慢性毒性。我们建议比较吸入铈、镍和银纳米颗粒对实验动物的急性和慢性毒性。这些具有商业重要性的纳米材料(和其他联盟选择的纳米材料)中的每一个都将为这些吸入研究动态生成。仅鼻子曝光系统已经建造完成，纳米颗粒生成系统几乎完成(截至2/11)。已完成的任务包括： 1.测试炉子系统以充分氧化老化的纳米颗粒 2.动物收容所内老龄系统的建设 3.订购用于纳米粒子生成的测试材料 4.招募一名接触评估的学生参与其硕士论文的研究

This research project focuses on the role of physical-chemical characteristics of nanoparticles in their interactions with cells, organelles, and organs after in vivo exposure via the inhalation route. During the first year, we will assess the biokinetics and toxicity of 3 selected nanoparticles under three different physical-chemical states. An important goal of our research will be the evaluation of acute vs. chronic toxicity of inhaled nanoparticles. We propose to compare the acute and chronic toxicity in test animals exposed by inhalation to cerium, nickel, and silver nanoparticles. Each of these commercially-important nanomaterials (and other Consortium-selected nanomaterials) will be dynamically generated for these inhalation studies. The nose-only exposure system has been constructed and the nanoparticle generation system is almost complete (as of 2/11). Tasks completed include: 1. testing of furnace system to fully oxidize aged nanoparticles 2. construction ofthe aging system in the animal housing facility 3. ordering of test material for nanoparticle generation 4. recruitment of an exposure assessment student to participate in the research for his Master's thesis