Epigenetics Core

表观遗传学核心

• 批准号: 7540231
• 负责人: MICHAEL A TEITELL
• 金额: $ 10.78万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7540231
• 关键词: Academia; Adopted; Adoption; Area; Bioinformatics; Biological Assay; Cell Count; Cells; Chromatin; Communities; Condition; Core Facility; Cytogenetics; DNA; DNA Methylation; Data; Data Collection; Detection; Epigenetic Process; Gene Expression; Gene Targeting; Genes; Genome; Genomics; Growth; Histone H3; Histones; Human Resources; Hybridization Array; Jonsson Comprehensive Cancer Center of University of California Los Angeles; Letters; Link; Measurement; Methods; Methylation; Modification; Molecular Profiling; Pan Genus; Pattern; Polymerase Chain Reaction; Procedures; Process; Protocols documentation; Publishing; Role; Sampling; Science; Services; Standards of Weights and Measures; Techniques; Technology; Training; Transcription Initiation Site; UC01; UC06; Validation; WA01 cell line; WA09 Cell Line; advanced system; analytical tool; bisulfite; chromatin immunoprecipitation; comparative; data acquisition; genome sequencing; human embryonic stem cell; interest; programs; promoter; technology development; tool development

The Epigenetics Core (Core C) provides genome-wide DMA methylation, histone modification, and chromatin immunoprecipitation (ChIP) services, along with targeted gene region analysis and validation, to support the Program Projects. The Epigenetics Core provides direct linkage of services to the Administrative Core A, hESC Core B, Computational/Bioinformatics Core D and also to essential existing UCLA cores, including the Gene Expression Core Facility (S Nelson). The Epigenetics Core is strongly supported by Agilent Technologies (for array platforms, probe technologies, and analytical tool development and adoption) and by key consultants that support Core services, including C Plass and T Huang (DMA methylation), M Grunstein and S Kurdistani (histone modification), and H Cedar (ChIP). Core C will provide genome-wide or targeted detection and validation for altered patterns of DNA methylation in hESCs and derivative differentiated cells from the hESC Core B and from Program Projects 1-3. Core C provides ChIP procedures, probe manufacture, array hybridizations, data collection, and analysis through transfer of data to Computational/ Bioinformatics Core D. The Epigenetics Core will distribute information and protocols and help train personnel (as needed) in each of the Program Projects and Cores and will have a limited but important role in technology development, focusing mainly on reducing cell number inputs for array ChlP-chip technologies with Core users and assessing/incorporating advances in epigenetic modification detection as they become available through the general scientific community and in conjunction with Agilent Technologies. Access to the Solexa 1G high throughput sequencing system for advanced ChlP-chip analysis is through the UCLA Gene Expression Core Facility (S Nelson). As an essential comparative service to the Program Project and to the hESC community as a whole, Epigenetics Core C will provide baseline measurements of global DNA methylation, pan-acetylated histone H3 and H4, and di- and tri-methylated histones, epigenetic markings that reflect active or silenced gene expression, for optimally grown low-passage federally-approved hESC lines UC 01 (HSF-1), UC 06 (HSF-6), WA01 (H1), and Wa09 (H9) for comparative analysis. Expression profiling for these 4 federally-approved lines (already published or to be performed in the existing UCLA Gene Expression Core Facility) will provide a link between epigenetic signatures and effects on hESC gene expression. This information will be made available in a publicly accessible portion of the Program Project Website, with data entry and manipulation password protected.

表观遗传学核心(核心C)提供全基因组的DNA甲基化、组蛋白修饰和染色质 免疫沉淀(CHIP)服务，以及目标基因区域分析和验证，以支持 计划项目。表观遗传学核心提供到管理核心A的服务的直接链接， HESC核心B、计算/生物信息学核心D以及必要的现有UCLA核心，包括 基因表达核心设施(S·尼尔森)。表观遗传学核心得到了安捷伦的大力支持 技术(用于阵列平台、探头技术以及分析工具的开发和采用)以及 支持核心服务的关键顾问，包括C Plass和T Huang(DMA甲基化)，M Grunstein 和S·库尔迪斯塔尼(组蛋白修饰)和H·雪松(CHIP)。核心C将提供全基因组或有针对性的 人类胚胎干细胞及其衍生分化细胞DNA甲基化改变模式的检测和验证 来自hESC核心B和计划项目1-3。核心C提供芯片程序、探头 制造、阵列杂交、数据收集和通过将数据传输到计算/ 生物信息学核心D.表观遗传学核心将分发信息和协议，并帮助培训 每个计划项目和核心中的人员(根据需要)，并将发挥有限但重要的作用 在技术开发方面，主要致力于减少阵列Chlp芯片技术的单元数输入 与核心用户合作，并评估/纳入表观遗传修饰检测方面的进展 可通过一般科学界以及与安捷伦技术公司联合使用。进入 用于高级ChlP芯片分析的Solexa 1G高通量测序系统是通过UCLA 基因表达核心设施(S·尼尔森)。作为对计划项目和 对于整个hESC社区，表观遗传学核心C将提供全球DNA的基线测量 甲基化，泛乙酰化组蛋白H3和H4，以及二甲基化和三甲基化的组蛋白，表观遗传标记 反映活跃或沉默的基因表达，用于联邦批准的最佳低传代hESC系 UC 01(HSF-1)、UC 06(HSF-6)、WA01(H1)和Wa09(H9)进行比较分析。表达特征分析 对于这4个联邦批准的品系(已经发表或将在现有的UCLA基因中执行 表达核心设备)将在表观遗传特征和对hESC基因的影响之间提供联系 表情。此信息将在计划项目的公开可访问部分中提供 网站，有数据输入和操作密码保护。