Parallel en-face optical coherence microscopy with adaptive focus
具有自适应焦点的并行正面光学相干显微镜
基本信息
- 批准号:8264194
- 负责人:
- 金额:$ 17.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-08-15 至 2015-04-30
- 项目状态:已结题
- 来源:
- 关键词:ArchitectureAutomobile DrivingBasement membraneBehaviorBiologicalBiological ModelsBiopsyCell physiologyCellsCellular MorphologyCheek structureClinicColonColon CarcinomaCorneaData CollectionDetectionDevelopmentDevicesDiagnosisDiagnosticDimensionsDiseaseElectronicsEpithelialEyeFaceFeedbackFrequenciesFutureGelatinGoalsHistologicImageImaging TechniquesIn SituIndividualLasersLeadLengthLifeLightLightingMechanicsMethodsMicroscopeMicroscopyMicrotomyMindMiniaturizationMovementNIH Program AnnouncementsNoiseOnionsOptical Coherence TomographyOpticsPerformancePhasePilot ProjectsProcessPublishingReaction TimeResearch Project GrantsResearch ProposalsResolutionSamplingScanningScreening for Oral CancerScreening procedureSignal TransductionSkinSpeedStructureSystemTadpolesTechniquesTechnologyTimeTissuesabstractingarmbasebioimagingcellular imagingdata acquisitiondesigndigitalimaging modalityinstrumentlensliquid crystalnovelocular surfaceportabilitypublic health relevanceresearch studytime usetissue phantomtoolvibrationvoltage
项目摘要
DESCRIPTION (provided by applicant): Parallel en-face optical coherence microscopy with adaptive focus Abstract. We propose a novel parallel nonstralational optical coherence microscopy (OCM) with adaptive focus for general high-speed high-resolution en-face biomedical imaging. Optical coherence tomography (OCT) has become an emerging imaging modality with high depth resolution. OCM is one kind of OCT imaging technique with better transverse and depth resolution by using confocal architecture in the object arm. Typical OCT systems generate B- scan cross section images. In clinic, the users are more familiar with C-scan (en face) images. Parallel OCT imaging has been studied, but not in the OCM architecture. Furthermore, in the conventional OCT/OCM imaging, good transverse resolution can be maintained only in the region close to the focal plane. Dynamic focusing can only be done by mechanic movement. To overcome these problems, here we propose to demonstrate, for the first time, a nontranslational parallel en-face OCM imaging instrument with adaptive focus. A programmable digital micromirror device is used for parallel confocal sampling, an electro-optic varifocal lens for fast depth scanning, and a rapid CMOS camera with more than 1000 frames/s rate for data collection. With adaptive focusing, the transverse resolution is constant across the depth of imaging. The spatial resolution can be around 2 <m W 2 <m W 4.5 <m in three dimensions. The imaging depth is higher than that of the confocal or full-field OCT imaging alone. With the rapid speed of the digital micromirror device (microsecond), the vafifocal lens, and the CMOS camera, the data acquisition speed is very high (volume/s). The pixel dwell time is increased. It allows lower excitation laser power and higher sensitivity. The signal-to-noise ratio would be higher than other full-field OCT imaging without confocal architecture. Since in the object arm, both longitudinal and transverse scanning are performed electro- optically without translational components, the moving effect of the sample due to mechanic vibration of the conventional imaging system can be avoided. The instrument provides a new tool to assess tissue and cell function and morphology in real time. In the proposed exploratory phase of this technology-driven project, we will perform experiments on general biologic relevant samples to demonstrate the functionality of the system. These samples include tissue phantoms based on gelatin embedded micro spheres covering a USAF 1951 target, onion skin, and tadpole. Images from biological samples will be compared to published OCT images and microscopy images of our corresponding thin sectioned samples. Pilot study of application of this imaging system for diagnosis of cornea disease will be performed too.
PUBLIC HEALTH RELEVANCE (provided by the applicant): We have proposed a novel parallel nonstralational optical coherence microscopy (OCM) with adaptive focus for general high-speed high-resolution en-face biomedical imaging. Our research proposal currently focuses on demonstration the feasibility of this new technique. With adaptive focusing, the transverse resolution is constant across the depth of imaging. [The spatial resolution can be around 2<m 4 2<m 4 4.5<m in three dimensions. The imaging depth is higher than that of the confocal or full-field OCT imaging alone. With the rapid speed of the digital micromirror device (microsecond), the varifocal lens, and the CMOS camera, the data acquisition speed is very high (~1190 frames/s or higher).It allows higher sensitivity. The signal-to-noise ratio would be higher than other full-field OCT imaging without confocal architecture. Since in the object arm, both longitudinal and transverse scannings are performed electro-optically without translational components, the moving effect of the sample due to mechanic vibration of the conventional imaging system can be avoided. The instrument provides a new tool to assess tissue and cell function and morphology in real time. The potential applications include general cell imaging, visualizing epithelial tissue layers or structures of the eye (cornea). Miniaturization and portability of the system will be considered in the future. Model system for epithelial tissue would be fresh samples of colon and cheek with the goal to image individual cells in epithelial tissues down to the basement membrane. This would demonstrate potential application in colon cancer screening and oral cancer screening. For eye imaging, such a system can be used for diagnosis of the ocular surface diseases.
描述(由申请人提供):具有自适应聚焦的平行面光学相干显微镜摘要。提出了一种新的具有自适应聚焦的并行非偏振光学相干显微镜(OCM),用于一般的高速高分辨率对面生物医学成像。光学相干层析成像(OCT)已成为一种新兴的成像模式,具有高的深度分辨率。光学共焦显微镜(OCM)是一种在物臂上采用共焦结构的光学相干断层成像技术,具有较好的横向和深度分辨率,典型的光学相干断层成像系统可获得B扫描截面图像。在临床上,用户更熟悉C扫描(正面)图像。并行OCT成像已被研究,但不是在OCM架构。此外,在传统的OCT/OCM成像中,仅在靠近焦平面的区域中可以保持良好的横向分辨率。动态聚焦只能通过机械运动来实现。为了克服这些问题,在这里,我们提出了证明,第一次,非平移平行面对OCM成像仪器与自适应聚焦。采用可编程数字CCD器件进行并行共焦采样,采用电光变焦透镜进行快速深度扫描,采用1000帧/秒以上的快速CMOS相机进行数据采集。利用自适应聚焦,横向分辨率在成像深度上是恒定的。在三维空间中,空间分辨率可以是大约2 <m W 2 <m W 4.5 <m。成像深度高于单独的共焦或全场OCT成像。由于数字图像处理装置(微秒)、变焦距透镜和CMOS照相机的快速,数据采集速度非常高(体积/秒)。像素停留时间增加。它允许较低的激发激光功率和较高的灵敏度。信噪比将高于没有共焦架构的其他全场OCT成像。由于在物臂中,纵向和横向扫描都是在没有平移分量的情况下电光地执行的,因此可以避免由于常规成像系统的机械振动而导致的样品的移动效应。该仪器为真实的实时评估组织和细胞的功能和形态提供了一种新的工具。在这个技术驱动项目的拟议探索阶段,我们将对一般生物相关样本进行实验,以证明系统的功能。这些样品包括基于明胶包埋微球的组织幻影,微球覆盖美国空军1951年的目标、洋葱皮和蝌蚪。将生物样本的图像与我们相应薄切片样本的已发表OCT图像和显微镜图像进行比较。本研究亦将进行此影像系统应用于角膜疾病诊断之初步研究。
公共卫生相关性(由申请人提供):我们提出了一种新的并行非分层光学相干显微镜(OCM),具有自适应聚焦,用于一般高速高分辨率的面生物医学成像。我们的研究计划目前集中在演示这种新技术的可行性。利用自适应聚焦,横向分辨率在成像深度上是恒定的。[The空间分辨率在三维中可以是大约2成像深度高于单独的共焦或全场OCT成像。由于数字摄像装置(微秒级)、变焦透镜、CMOS摄像机的高速化,数据采集速度非常快(~1190帧/秒以上),因此可以实现更高的灵敏度。信噪比将高于没有共焦架构的其他全场OCT成像。由于在物体臂中,纵向和横向扫描都是在没有平移分量的情况下电光地执行的,因此可以避免由于常规成像系统的机械振动而导致的样品的移动效应。该仪器为真实的实时评估组织和细胞的功能和形态提供了一种新的工具。潜在的应用包括一般细胞成像,可视化上皮组织层或眼睛(角膜)的结构。今后将考虑该系统的小型化和便携性。用于上皮组织的模型系统将是结肠和脸颊的新鲜样本,目标是对上皮组织中的单个细胞进行成像,直到基底膜。这将证明在结肠癌筛查和口腔癌筛查中的潜在应用。对于眼睛成像,这样的系统可以用于眼表面疾病的诊断。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Guoqiang Li其他文献
Guoqiang Li的其他文献
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{{ truncateString('Guoqiang Li', 18)}}的其他基金
Parallel en-face optical coherence microscopy with adaptive focus
具有自适应焦点的并行正面光学相干显微镜
- 批准号:
7946988 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Electro-optic adaptive eyeglass for correction of presbyopia
矫正老花眼的电光自适应眼镜
- 批准号:
8717666 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Electro-optic adaptive eyeglass for correction of presbyopia
矫正老花眼的电光自适应眼镜
- 批准号:
7993737 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Parallel en-face optical coherence microscopy with adaptive focus
具有自适应焦点的并行正面光学相干显微镜
- 批准号:
8514371 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Electro-optic adaptive eyeglass for correction of presbyopia
矫正老花眼的电光自适应眼镜
- 批准号:
8278640 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Electro-optic adaptive eyeglass for correction of presbyopia
矫正老花眼的电光自适应眼镜
- 批准号:
8138407 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Electro-optic adaptive eyeglass for correction of presbyopia
矫正老花眼的电光自适应眼镜
- 批准号:
8549251 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Parallel en-face optical coherence microscopy with adaptive focus
具有自适应焦点的并行正面光学相干显微镜
- 批准号:
8122323 - 财政年份:2010
- 资助金额:
$ 17.3万 - 项目类别:
Rapid parallel 3D confocal/fluorescence cell imaging with varifocal lens
使用变焦镜头进行快速并行 3D 共焦/荧光细胞成像
- 批准号:
7880578 - 财政年份:2009
- 资助金额:
$ 17.3万 - 项目类别:
Rapid parallel 3D confocal/fluorescence cell imaging with varifocal lens
使用变焦镜头进行快速并行 3D 共焦/荧光细胞成像
- 批准号:
8471234 - 财政年份:2009
- 资助金额:
$ 17.3万 - 项目类别:
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