Parallel en-face optical coherence microscopy with adaptive focus

具有自适应焦点的并行正面光学相干显微镜

• 批准号: 8122323
• 负责人: Guoqiang Li
• 金额: $ 3.92万
• 依托单位: UNIVERSITY OF MISSOURI-ST. LOUIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8122323
• 关键词: Architecture; Automobile Driving; Basement membrane; Behavior; Biological; Biological Models; Biopsy; Cell physiology; Cells; Cellular Morphology; Cheek structure; Clinic; Colon; Colon Carcinoma; Cornea; Data Collection; Detection; Development; Devices; Diagnosis; Diagnostic; Dimensions; Disease; Electronics; Epithelial; Eye; Face; Feedback; Frequencies; Future; Gelatin; Goals; Histologic; Image; Imaging Techniques; In Situ; Individual; Lasers; Lead; Length; Life; Light; Lighting; Mechanics; Methods; Microscope; Microscopy; Microtomy; Mind; Miniaturization; Movement; NIH Program Announcements; Noise; Onions; Optical Coherence Tomography; Optics; Performance; Phase; Pilot Projects; Process; Publishing; Reaction Time; Research Project Grants; Research Proposals; Resolution; Sampling; Scanning; Screening for Oral Cancer; Screening procedure; Signal Transduction; Skin; Speed; Structure; System; Tadpoles; Techniques; Technology; Time; Tissues; abstracting; arm; base; bioimaging; cellular imaging; data acquisition; design; digital; imaging modality; instrument; lens; liquid crystal; novel; ocular surface; portability; public health relevance; research study; time use; tissue phantom; tool; vibration; voltage

DESCRIPTION (provided by applicant): Parallel en-face optical coherence microscopy with adaptive focus Abstract. We propose a novel parallel nonstralational optical coherence microscopy (OCM) with adaptive focus for general high-speed high-resolution en-face biomedical imaging. Optical coherence tomography (OCT) has become an emerging imaging modality with high depth resolution. OCM is one kind of OCT imaging technique with better transverse and depth resolution by using confocal architecture in the object arm. Typical OCT systems generate B- scan cross section images. In clinic, the users are more familiar with C-scan (en face) images. Parallel OCT imaging has been studied, but not in the OCM architecture. Furthermore, in the conventional OCT/OCM imaging, good transverse resolution can be maintained only in the region close to the focal plane. Dynamic focusing can only be done by mechanic movement. To overcome these problems, here we propose to demonstrate, for the first time, a nontranslational parallel en-face OCM imaging instrument with adaptive focus. A programmable digital micromirror device is used for parallel confocal sampling, an electro-optic varifocal lens for fast depth scanning, and a rapid CMOS camera with more than 1000 frames/s rate for data collection. With adaptive focusing, the transverse resolution is constant across the depth of imaging. The spatial resolution can be around 2 <m W 2 <m W 4.5 <m in three dimensions. The imaging depth is higher than that of the confocal or full-field OCT imaging alone. With the rapid speed of the digital micromirror device (microsecond), the vafifocal lens, and the CMOS camera, the data acquisition speed is very high (volume/s). The pixel dwell time is increased. It allows lower excitation laser power and higher sensitivity. The signal-to-noise ratio would be higher than other full-field OCT imaging without confocal architecture. Since in the object arm, both longitudinal and transverse scanning are performed electro- optically without translational components, the moving effect of the sample due to mechanic vibration of the conventional imaging system can be avoided. The instrument provides a new tool to assess tissue and cell function and morphology in real time. In the proposed exploratory phase of this technology-driven project, we will perform experiments on general biologic relevant samples to demonstrate the functionality of the system. These samples include tissue phantoms based on gelatin embedded micro spheres covering a USAF 1951 target, onion skin, and tadpole. Images from biological samples will be compared to published OCT images and microscopy images of our corresponding thin sectioned samples. Pilot study of application of this imaging system for diagnosis of cornea disease will be performed too. PUBLIC HEALTH RELEVANCE (provided by the applicant): We have proposed a novel parallel nonstralational optical coherence microscopy (OCM) with adaptive focus for general high-speed high-resolution en-face biomedical imaging. Our research proposal currently focuses on demonstration the feasibility of this new technique. With adaptive focusing, the transverse resolution is constant across the depth of imaging. [The spatial resolution can be around 2<m 4 2<m 4 4.5<m in three dimensions. The imaging depth is higher than that of the confocal or full-field OCT imaging alone. With the rapid speed of the digital micromirror device (microsecond), the varifocal lens, and the CMOS camera, the data acquisition speed is very high (~1190 frames/s or higher).It allows higher sensitivity. The signal-to-noise ratio would be higher than other full-field OCT imaging without confocal architecture. Since in the object arm, both longitudinal and transverse scannings are performed electro-optically without translational components, the moving effect of the sample due to mechanic vibration of the conventional imaging system can be avoided. The instrument provides a new tool to assess tissue and cell function and morphology in real time. The potential applications include general cell imaging, visualizing epithelial tissue layers or structures of the eye (cornea). Miniaturization and portability of the system will be considered in the future. Model system for epithelial tissue would be fresh samples of colon and cheek with the goal to image individual cells in epithelial tissues down to the basement membrane. This would demonstrate potential application in colon cancer screening and oral cancer screening. For eye imaging, such a system can be used for diagnosis of the ocular surface diseases.

描述(申请人提供)：具有自适应聚焦的并行面上光学相干显微镜摘要。我们提出了一种新型的具有自适应焦距的并行非层状光学相干显微镜(OCM)，用于普通高速高分辨率的面部生物医学成像。光学相干层析成像(OCT)已成为一种新兴的高深度分辨率成像方式。OCM是一种在物臂上采用共焦结构的OCT成像技术，具有较好的横向和深度分辨率。典型的OCT系统生成B超横断面图像。在临床上，用户对C-Scan(En Face)图像更加熟悉。并行OCT成像已经被研究过了，但在OCM体系结构中还没有。此外，在传统的OCT/OCM成像中，只能在靠近焦平面的区域保持良好的横向分辨率。动态对焦只能通过机械运动来完成。为了克服这些问题，我们首次提出了一种具有自适应聚焦的非平移平行内脸OCM成像仪。采用可编程数字微镜进行并行共焦采样，采用电光变焦透镜进行快速深度扫描，采用1000帧/S速率以上的快速CMOS机进行数据采集。使用自适应对焦，横向分辨率在成像深度上是恒定的。三维空间分辨率可在2&lt；m W 2&lt；m W 4.5&lt；m左右。成像深度高于共焦或全视场OCT成像。随着数字微镜器件(微秒)、变焦镜头和cmos相机的快速运行，数据采集速度非常快(体积/S)。像素驻留时间增加。它允许较低的激励激光功率和较高的灵敏度。其信噪比将高于其他无共焦结构的全场OCT成像。由于在物臂中，纵向和横向扫描都是在没有平移部件的情况下进行电光扫描的，因此可以避免由于传统成像系统的机械振动而引起的样品的移动效应。该仪器为实时评估组织和细胞的功能和形态提供了一种新的工具。在这个技术驱动的项目的拟议探索阶段，我们将对一般生物相关样本进行实验，以演示系统的功能。这些样本包括基于明胶嵌入微球的组织模体，覆盖了美国空军1951年的靶子、洋葱皮和蝌蚪。来自生物样本的图像将与发表的OCT图像和我们相应的薄片样本的显微图像进行比较。该成像系统在角膜疾病诊断中的应用也将进行初步研究。 公共卫生相关性(由申请人提供)：我们提出了一种新的具有自适应聚焦的平行非层状光学相干显微镜(OCM)，用于普通高速高分辨率面部生物医学成像。我们的研究方案目前集中在论证这项新技术的可行性。使用自适应对焦，横向分辨率在成像深度上是恒定的。[三维空间分辨率可在2&lt；m42&lt；m44.5&lt；m左右。成像深度高于共焦或全视场OCT成像。由于数字微镜器件(微秒)、变焦镜头和cmos相机的速度很快，数据采集速度非常快(~1190帧/S或更高)，允许更高的灵敏度。其信噪比将高于其他无共焦结构的全场OCT成像。由于在物臂中，纵向和横向扫描都是在没有平移部件的情况下进行电光扫描的，因此可以避免由于传统成像系统的机械振动而引起的样品的移动效应。该仪器为实时评估组织和细胞的功能和形态提供了一种新的工具。潜在的应用包括一般的细胞成像，可视化上皮组织层或眼睛(角膜)的结构。未来将考虑系统的小型化和便携性。上皮组织的模型系统将是新鲜的结肠和脸颊样本，目标是对上皮组织中的单个细胞进行成像，直到基底膜。这将展示其在结肠癌筛查和口腔癌筛查中的潜在应用。对于眼部成像，该系统可用于眼表疾病的诊断。