Characterization of the LGL Leukemia Virus (PQ 12)

LGL 白血病病毒 (PQ 12) 的特征

基本信息

批准号：
8383318
负责人：
Thomas Patrick Loughran
金额：
$ 54.81万
依托单位：
PENNSYLVANIA STATE UNIV HERSHEY MED CTR
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-17 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8383318
关键词：
Address Affect Animals Antibodies Antigens Aplastic Anemia Biological Assay Blood Transfusion Cell Line Cells Child Coculture Techniques Custom Cytotoxic T-Lymphocytes DNA Data Development Disease Dysmyelopoietic Syndromes Electron Microscopy Enzyme-Linked Immunosorbent Assay Etiology Failure Family member Gagging Genes Genome Genomics Giant Cells Goals HIV Horizontal Disease Transmission Human Human T-Cell Leukemia Viruses Imagery Individual Infection Infectious Agent Laboratories Large granular lymphocyte Leukemic Cell Link Malignant Neoplasms Marrow Methodology Methods Molecular Morphology Normal Cell Oligonucleotides Patients Pattern Peptides Plants Preventive Production Pure Red-Cell Aplasia RNA-Directed DNA Polymerase Reagent Retroviridae Rheumatoid Arthritis Risk Role Satellite Viruses Screening procedure Serological Serum Signal Transduction Site Spouses Testing Therapeutic Transcript Vaccines Vertical Disease Transmission Viral Virus Work anticancer research authority chronic T-cell leukemia design experience human disease leukemia leukemia virus member next generation novel particle prevent rat Piga protein transmission process tumor virology

项目摘要

DESCRIPTION (provided by applicant): The long-term goal of this project is to identify the virus associated with LGL leukemia. This application represents a combination of classical and cutting edge approaches led by a multi-disciplinary team whose members are internationally recognized for their individual contributions to cancer research and mammalian genomics. We propose that LGL leukemia is caused by a novel retrovirus with similarities to both HTLV and HIV. We will use classical virology methods to isolate the LGL leukemia virus. We will test the hypothesis that the LGL virus can be transmitted from infected cells to target cells and identify optimum targets for high titer virus production. We will use state-of-the-art deep sequencing combined with whole genome analytical approaches to identify LGL leukemia viral sequences. PUBLIC HEALTH RELEVANCE: This study is investigating the role of a virus in LGL leukemia development. The purpose of this study is to identify the LGL leukemia virus and to understand how it might cause leukemia. This information will make it easier to detect people who are at risk for LGL leukemia, to prevent LGL leukemia and to better treat patients who are affected by the LGL leukemia virus. It will also allow us to determine if the LGL leukemia virus is involved in other cancers and diseases with features that are similar to LGL leukemia.

描述（由申请人提供）：该项目的长期目标是识别与LGL白血病相关的病毒。该应用代表了由多学科团队领导的经典和前沿方法的结合，该团队的成员因其对癌症研究和哺乳动物基因组学的个人贡献而受到国际认可。我们建议LGL白血病是由一种与HTLV和HIV相似的新型逆转录病毒引起的。我们将使用经典的病毒学方法分离LGL白血病病毒。我们将检验以下假设：LGL病毒可以从感染细胞传播到靶细胞，并确定高滴度病毒产生的最佳靶标。我们将使用最新的深层测序与整个基因组分析方法结合使用，以鉴定LGL白血病病毒序列。公共卫生相关性：这项研究正在研究病毒在LGL白血病开发中的作用。这项研究的目的是鉴定LGL白血病病毒，并了解其可能导致白血病。这些信息将使检测患有LGL白血病风险的人，以防止LGL白血病并更好地治疗受LGL白血病病毒影响的患者。它还使我们能够确定LGL白血病病毒是否参与其他具有类似于LGL白血病的癌症和疾病。