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Characterization of the LGL Leukemia Virus (PQ 12)

LGL 白血病病毒 (PQ 12) 的特征

基本信息

批准号：
8546319
负责人：
Thomas Patrick Loughran
金额：
$ 50.61万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-09-17 至 2015-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8546319
关键词：
Address Affect Animals Antibodies Antigens Aplastic Anemia Biological Assay Blood Transfusion Cell Line Cells Child Coculture Techniques Custom Cytotoxic T-Lymphocytes DNA Data Development Disease Dysmyelopoietic Syndromes Electron Microscopy Enzyme-Linked Immunosorbent Assay Etiology Failure Family member Gagging Genes Genome Genomics Giant Cells Goals HIV Horizontal Disease Transmission Human Human T-Cell Leukemia Viruses Imagery Individual Infection Infectious Agent Laboratories Large granular lymphocyte Leukemic Cell Link Malignant Neoplasms Marrow Methodology Methods Molecular Morphology Normal Cell Oligonucleotides Patients Pattern Peptides Plants Preventive Production Pure Red-Cell Aplasia RNA-Directed DNA Polymerase Reagent Retroviridae Rheumatoid Arthritis Risk Role Satellite Viruses Serological Serum Signal Transduction Site Spouses Testing Therapeutic Transcript Vaccines Vertical Disease Transmission Viral Virus Work anticancer research authority chronic T-cell leukemia deep sequencing design experience human disease leukemia leukemia virus member next generation sequencing novel particle prevent rat Piga protein screening transmission process tumor virology

项目摘要

DESCRIPTION (provided by applicant): The long-term goal of this project is to identify the virus associated with LGL leukemia. This application represents a combination of classical and cutting edge approaches led by a multi-disciplinary team whose members are internationally recognized for their individual contributions to cancer research and mammalian genomics. We propose that LGL leukemia is caused by a novel retrovirus with similarities to both HTLV and HIV. We will use classical virology methods to isolate the LGL leukemia virus. We will test the hypothesis that the LGL virus can be transmitted from infected cells to target cells and identify optimum targets for high titer virus production. We will use state-of-the-art deep sequencing combined with whole genome analytical approaches to identify LGL leukemia viral sequences.

描述（由申请人提供）：该项目的长期目标是鉴定与LGL白血病相关的病毒。该应用程序代表了由一个多学科团队领导的经典和前沿方法的结合，该团队的成员因其对癌症研究和哺乳动物基因组学的个人贡献而受到国际认可。我们提出LGL白血病是由一种与HTLV和HIV相似的新型逆转录病毒引起的。我们将使用经典病毒学方法分离LGL白血病病毒。我们将验证LGL病毒可以从感染细胞传播到靶细胞的假设，并确定高滴度病毒生产的最佳靶点。我们将使用最先进的深度测序结合全基因组分析方法来鉴定LGL白血病病毒序列。