Small-Animal Optical Imaging System

描述（由申请人提供）：本提案的目标是获得资金，为分子和基因组成像中心增加IVIS光谱光学成像系统，该中心是加州大学戴维斯分校的核心设施，提供进行体内成像的基础设施和专业知识 动物模型的研究。IVIS Spectrum（Caliper Life Sciences）将用于携带荧光素酶或荧光报告基因的细胞和组织以及注射荧光成像剂或放射性药物的动物的体内成像研究，后者通过发射Cerenkov光进行研究。IVIS Spectrum系统可用于反射和透射模式，包括一个高灵敏度的背照式1级CCD相机、一个带荧光测量滤光片的宽带激发源、用于多光谱检测的可选滤光片、一个带麻醉供应的加热外壳以及用于控制数据采集的软件，用于光谱分解、3-D信号定位和定量，以及图像显示和分析。该系统将被放置在我们的核心设施中，用于支持和帮助NIH资助的癌症、动脉粥样硬化、伤口愈合和炎症等不同领域的研究，以及评估靶向分子成像探针和治疗开发以及药物输送技术的大量研究。该系统还将作为进一步开发和应用我们机构正在开发的新型切伦科夫成像技术的平台。总之，该系统将直接支持新的靶向诊断和治疗策略的开发，以及表征和更好地理解人类疾病动物模型中正常和患病组织的分子和生化基础。 公共卫生相关性：分子成像领域寻求发现体内成像特定生物靶点和途径的新方法，最终目标是提供患者特异性和基于分子的诊断信息。此外，正在开发分子成像方法以直接监测分子靶向治疗、细胞治疗和基因治疗。拟议的IVIS光谱仪器将用于研究整个动物中荧光分子、颗粒和光学（生物发光和荧光）报告蛋白的空间和时间分布。这提供了一个强大的平台，用于表征动物疾病模型，并在临床前模型中非侵入性地确定新诊断和治疗策略的有效性。

DESCRIPTION (provided by applicant): The goal of this proposal is to secure funding to add an IVIS Spectrum optical imaging system to the Center for Molecular and Genomic Imaging, a core facility at UC Davis that provides the infrastructure and expertise to conduct in vivo imaging studies in animal models. The IVIS Spectrum (Caliper Life Sciences) will be used for in vivo imaging studies of cells and tissues carrying luciferase or fluorescent reporter genes, and animals injected with fluorescent imaging agents or radiopharmaceuticals, the latter being studied by the emission of Cerenkov light. The IVIS Spectrum system can be used in both reflectance and transmission mode and includes a highly sensitive back-thinned, back-illuminated grade 1 CCD camera, a broad band excitation source with filters for fluorescence measurements, selectable filters for multispectral detection, a heated enclosure with anesthesia supply, and software to control data acquisition, for spectral unmixing, 3-D signal localization and quantification, and image display and analysis. The system will be placed in our core facility and used to support and aid NIH-funded research in diverse areas such as cancer, atherosclerosis, wound healing, and inflammation, and in a large body of research to evaluate targeted molecular imaging probe and therapeutic development, as well as drug delivery technologies. The system also will serve as the platform for further development and application of novel Cerenkov imaging techniques being developed at our institution. In summary, this system will directly support the development of new targeted diagnostic and therapeutic strategies, as well as to characterize and better understand the molecular and biochemical basis of normal and diseased tissue in animal models of human disease. PUBLIC HEALTH RELEVANCE: The field of molecular imaging seeks to discover new approaches to imaging specific biologic targets and pathways in vivo, with the ultimate goal of providing patient-specific and molecularly-based diagnostic information. In addition, molecular imaging approaches are being developed to directly monitor molecularly targeted therapies, cellular therapies and gene therapies. The proposed IVIS Spectrum instrument will be used to study the spatial and temporal distribution of fluorescent molecules, particles, and optical (bioluminescent and fluorescent) reporter proteins in the whole animal. This provides a powerful platform for characterizing animal disease models and non-invasively determining the efficacy of new diagnostic and therapeutic strategies in preclinical models prior to translation to the clinic.