Proteomics, Metabolomics and Lipidomics

蛋白质组学、代谢组学和脂质组学

• 批准号: 8580047
• 负责人: RICHARD D SMITH
• 金额: $ 124.8万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8580047
• 关键词: Biological Process; Computer Simulation; Data; Ebola virus; Gene Targeting; Human Virus; Immune response; Infection; Influenza; Influenza A virus; Instruction; Knowledge; Laboratories; Lipids; Mass Spectrum Analysis; Measurement; Modeling; Molecular Profiling; Pacific Northwest; Phosphopeptides; Process; Proteins; Proteomics; Resolution; Resources; Sample Size; Stable Isotope Labeling; Support System; Systems Biology; Time; Validation; Virus Diseases; West Nile viral infection; West Nile virus; base; biological systems; combat; global health; insight; metabolomics; pathogen; programs; protein metabolite; stable isotope; transcriptomics

PROJECT SUMMARY (See instructions): The Proteomics, Metabolomics, and Lipidomics Core will be located at Pacific Northwest National Laboratory (PNNL). PNNL OMICs Core measurements will be based upon world-class mass spectrometry (MS)-based platforms and the availability of unique capabilities and resources. Ultra-sensitive proteomics analyses will be performed using the accurate mass and time (AMT) tag approach together with high resolution mass spectrometry, a process that offers high OMIC coverage, high quantitative precision, and high sensitivity with small sample-size requirements. Quantification of global phosphopeptide measurements will be obtained using ITRAQ stable isotope labeling. The global metabolomics and lipidomics components of this Core will utilize GC-MS and LC-MS, respectively. These studies will enhance our understanding of the host response to infection by evaluating changes in the abundance for the broad range of host proteins, metabolites, and lipid molecules that directly carry out biological functions. Based on the results of complementary transcriptomics (analyzed in parallel outside of this Core) and global MS-based OMICs data or a priori knowledge of the biological systems under study, we will also then employ multiplexed targeted MS-based analyses for quantifying a defined number of substrates and/or products of corresponding encoded proteins. These targeted proteomics, metabolomics and lipidomics analyses will provide accurate absolute quantification based on stable-isotope dilution where feasible

项目概述（见说明）：蛋白质组学、代谢组学和脂质组学核心将位于太平洋西北国家实验室（PNNL）。PNNL组学核心测量将基于世界级的质谱（MS）平台和独特的能力和资源的可用性。超灵敏的蛋白质组学分析将使用精确质量和时间（AMT）标签方法以及高分辨率质谱法进行，该方法具有高OMIC覆盖率，高定量精度和高灵敏度，且样本量要求小。全球磷酸肽测量的定量将获得使用ITRAQ稳定同位素标记。该核心的全球代谢组学和脂质组学成分将分别使用GC-MS和LC-MS。这些研究将通过评估宿主蛋白、代谢物和脂质分子丰度的变化来增强我们对宿主对感染的反应的理解，这些蛋白、代谢物和脂质分子直接执行生物功能。基于互补转录组学的结果（在本核心之外并行分析）和全球MS-based组学数据或对所研究生物系统的先验知识，我们还将采用多路靶向MS-based分析来定量定义数量的底物和/或相应编码蛋白的产物。这些靶向蛋白质组学、代谢组学和脂质组学分析将在可行的情况下提供基于稳定同位素稀释的准确绝对定量