Proteomics, Metabolomics and Lipidomics

蛋白质组学、代谢组学和脂质组学

• 批准号: 8580047
• 负责人: RICHARD D SMITH
• 金额: $ 124.8万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8580047
• 关键词: Biological Process; Computer Simulation; Data; Ebola virus; Gene Targeting; Human Virus; Immune response; Infection; Influenza; Influenza A virus; Instruction; Knowledge; Laboratories; Lipids; Mass Spectrum Analysis; Measurement; Modeling; Molecular Profiling; Pacific Northwest; Phosphopeptides; Process; Proteins; Proteomics; Resolution; Resources; Sample Size; Stable Isotope Labeling; Support System; Systems Biology; Time; Validation; Virus Diseases; West Nile viral infection; West Nile virus; base; biological systems; combat; global health; insight; metabolomics; pathogen; programs; protein metabolite; stable isotope; transcriptomics

PROJECT SUMMARY (See instructions): The Proteomics, Metabolomics, and Lipidomics Core will be located at Pacific Northwest National Laboratory (PNNL). PNNL OMICs Core measurements will be based upon world-class mass spectrometry (MS)-based platforms and the availability of unique capabilities and resources. Ultra-sensitive proteomics analyses will be performed using the accurate mass and time (AMT) tag approach together with high resolution mass spectrometry, a process that offers high OMIC coverage, high quantitative precision, and high sensitivity with small sample-size requirements. Quantification of global phosphopeptide measurements will be obtained using ITRAQ stable isotope labeling. The global metabolomics and lipidomics components of this Core will utilize GC-MS and LC-MS, respectively. These studies will enhance our understanding of the host response to infection by evaluating changes in the abundance for the broad range of host proteins, metabolites, and lipid molecules that directly carry out biological functions. Based on the results of complementary transcriptomics (analyzed in parallel outside of this Core) and global MS-based OMICs data or a priori knowledge of the biological systems under study, we will also then employ multiplexed targeted MS-based analyses for quantifying a defined number of substrates and/or products of corresponding encoded proteins. These targeted proteomics, metabolomics and lipidomics analyses will provide accurate absolute quantification based on stable-isotope dilution where feasible

项目摘要（见说明）：蛋白质组学、代谢组学和脂质组学核心将位于太平洋西北国家实验室（PNNL）。PNNL OMIC核心测量将基于世界一流的质谱（MS）平台以及独特的能力和资源。超灵敏蛋白质组学分析将使用准确的质量和时间（AMT）标签方法以及高分辨率质谱法进行，该方法提供高OMIC覆盖率，高定量精度和高灵敏度以及小样本量要求。将使用ITRAQ稳定同位素标记获得全局磷酸肽测量的定量。该核心的总体代谢组学和脂质组学组件将分别使用GC-MS和LC-MS。这些研究将通过评估直接发挥生物学功能的广泛宿主蛋白质、代谢物和脂质分子丰度的变化，增强我们对宿主感染反应的理解。基于互补转录组学的结果（在本核心之外并行分析）和基于MS的全局OMIC数据或所研究的生物系统的先验知识，我们还将采用基于MS的多重靶向分析来定量相应编码蛋白的限定数量的底物和/或产物。这些有针对性的蛋白质组学、代谢组学和脂质组学分析将在可行的情况下基于稳定同位素稀释提供准确的绝对定量