APPROACHES FOR PROTEIN MODIFICATIONS, INTERACTIONS, & SPATIAL & QUANTITATIVE DYN

蛋白质修饰、相互作用的方法，

• 批准号: 8365459
• 负责人: RICHARD D SMITH
• 金额: $ 34.41万
• 依托单位: BATTELLE PACIFIC NORTHWEST LABORATORIES
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8365459
• 关键词: Annual Reports; Area; Biology; Cell Fractionation; Cell surface; Cells; Development; Digestion; Fractionation; Funding; Gases; Genome; Goals; Grant; Measurement; Measures; Membrane; Methods; Modification; National Center for Research Resources; Oncogenic Viruses; Phase; Post-Translational Protein Processing; Preparation; Principal Investigator; Proteins; Proteome; Proteomics; Reporting; Research; Research Infrastructure; Research Personnel; Resources; Saliva; Sampling; Sonication; Source; Sperm Maturation; Subcellular Fractions; Systems Biology; Tissues; Trypsin; United States National Institutes of Health; base; cost; nanoparticle; pressure; protein protein interaction; protein transport; trafficking; viral detection

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core 1 focuses on developments associated protein localization within the cell, the protein-protein interactions, the quantitative dynamics and protein trafficking, and the modification states of proteins applying high throughput proteomics measurements. For this annual report advancements in top-down proteomics by Center-investigators are provided; advancement in applying gas phase fractionation with FAIMS are described; measurements of posttranslational modifications including the phosphoproteome with measurements of low abundance pTyr modifications in addition to the more abundant pSer and pThr modifications; and an enhanced version of RePlay proteomics is reported. Advanced methods of sample preparation (e.g., trypsin coated nanoparticles; the biofluids CSF and saliva), pressure digestion and sonication, have been developed in support of measuring differential protein abundances for systems biology studies. Core 1 also includes the research area of activity-based proteomics (ABP and MABPP) that provides a more targeted approach to probing the sub-proteomes of tissues, cells, subcellular fractions, and biofluids. Methods in subcellular fractionation are being implemented in the Center to achieve Core 1 goals for characterizing proteins in cells and their associated localization and trafficking. Additional progress has been made on profiling the cell surface membrane proteome, sperm maturation, oncogenic virus detection, and genome annotation.

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