Gametocyte Carriage Rate and Transmission Blocking Vaccine Assay Development

采用嵌套式病例对照队列设计，将马里班库马纳疟疾暴露人群的蚊子喂养试验方法的结果与在NIH校园使用培养的寄生虫进行的标准膜喂养试验进行比较。班古马纳村庄的500名年龄在3个月到50岁之间的志愿者被同意并登记在册。每月访查疟疾寄生虫携带率，包括无性期和配子体阶段。配子体阳性个体和配子体阴性个体被要求参加用昆虫饲养的蚊子的喂养实验。将这些蚊子的传染性与马里和美国的薄膜喂养蚊子的传染性进行了比较。寄生虫携带率数据和蚊子感染数据被用于指导研究设计，以评估目标人群中的传播阻断疫苗。

Using a nested case-control cohort design, results of mosquito feeding assay methods in a malaria exposed population in Bancoumana, Mali will be compared with standard membrane feeding assay conducted at the NIH campus using cultured parasites. 500 volunteers, ages 3 months to 50 years in Bancoumana villages, are consented and enrolled. Malaria parasite carriage rate, including asexual stage and gametocyte stage are survey at monthly visits. Gametocytemic individuals and gametocyte negative individuals are asked to participate in feed experiments using insectary-raised mosquitoes. Infectivity in these mosquitoes are compared against those of mosquitoes fed in membrane feeding assays in Mali and the USA. The parasite carriage rate data and mosquito infection data are used to inform study design to evaluate transmission blocking vaccine in target populations.