Gametocyte Carriage Rate and Transmission Blocking Vaccine Assay Development

配子体携带率和传播阻断疫苗检测的开发

• 批准号: 8745591
• 负责人: Patrick Duffy
• 金额: $ 117.68万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8745591
• 关键词: Age; Antibodies; Baseline Surveys; Biological Assay; Consent; Culicidae; Data; Development; Disease; Enrollment; Evaluation; Goals; Human; Individual; Infection; Malaria; Mali; Membrane; Methods; Parasites; Population; Qualifying; Research Design; Site; Staging; Surveys; Target Populations; United States National Institutes of Health; Vaccinated; Vaccines; Visit; Work; asexual; assay development; case control; cohort; design; efficacy evaluation; feeding; research study; tool; transmission process; vaccine candidate; vaccine efficacy; volunteer

Using a nested case-control cohort design, results of mosquito feeding assay methods in a malaria exposed population in Bancoumana, Mali will be compared with standard membrane feeding assay conducted at the NIH campus using cultured parasites. 500 volunteers, ages 3 months to 50 years in Bancoumana villages, are consented and enrolled. Malaria parasite carriage rate, including asexual stage and gametocyte stage are survey at monthly visits. Gametocytemic individuals and gametocyte negative individuals are asked to participate in feed experiments using insectary-raised mosquitoes. Infectivity in these mosquitoes are compared against those of mosquitoes fed in membrane feeding assays in Mali and the USA. The parasite carriage rate data and mosquito infection data are used to inform study design to evaluate transmission blocking vaccine in target populations.

采用巢式病例对照队列设计，将在马里贝塔斯曼疟疾暴露人群中进行的蚊子喂养试验方法的结果与在NIH校园使用培养的寄生虫进行的标准膜喂养试验进行比较。 在Baghmana村庄，有500名年龄在3个月到50岁之间的志愿者同意并登记。疟疾寄生虫携带率，包括无性阶段和配子体阶段，每月进行一次调查。要求配子母细胞血症个体和配子母细胞阴性个体参与使用昆虫饲养的蚊子的饲料实验。这些蚊子的繁殖力与在马里和美国的膜喂养试验中喂养的蚊子进行了比较。寄生虫携带率数据和蚊子感染数据用于为研究设计提供信息，以评价目标人群中的传播阻断疫苗。