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Malaria Vaccine: Pfs230

Malaria Vaccine: Pfs230
疟疾疫苗：Pfs230
批准号：
8745458
负责人：
Patrick Duffy
金额：
$168.11万
依托单位：
NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/8745458
关键词：
AnimalsAntibodiesAreaBiological AssayComplementCyclic GMPDataEscherichia coliGermGoalsHumanImmune SeraMalariaMalaria VaccinesMembraneParasitesPichiaPopulationProductionProteinsRecombinantsSurfaceVaccinesWheatbaseburden of illnessexpression cloningfeedingsuccesstransmission processvaccine evaluation

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中文摘要
翻译
在成功评估小麦胚芽表达的Pfs230结构域的基础上，重组Pfs230结构域也在巴氏酵母和大肠杆菌中表达。在离体标准膜喂养试验中，以互补依赖的方式，针对这些结构域提出的抗血清显示出有效的传输阻断活性。评估多个表达克隆对重组Pfs230的产率和诱导功能抗体的影响。选择毕赤酵母表达克隆，制备cGMP级重组Pfs230。目前的工作重点是生产cGMP级Pfs230-EPA，与Pfs25-EPA疫苗联合使用，并作为潜在有效的传播阻断疫苗在人体中进行测试。
英文摘要
Built on success in evaluating the wheat germ-expressed Pfs230 domains, recombinant Pfs230 domains were also expressed in P. pastoris and E. coli. Antisera raised against these domains demonstrated potent transmission blocking activities in an ex vivo standard membrane feeding assay, in a complement-dependent manner. Multiple expression clones were evaluated for their recombinant Pfs230 production yield and induction of functional antibodies. A Pichia expression clone was selected, and cGMP grade recombinant Pfs230 was manufactured. The current efforts are focused on generating cGMP grade Pfs230-EPA, to be combined with Pfs25-EPA vaccine and tested in humans as potentially effective transmission blocking vaccine.
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会议论文
Malaria Surveillance and Research Studies in Liberia and Guinea-Conakry
Malaria Transmission Blocking Vaccine Discovery
Malaria Pathogenesis in young children and vaccine discovery
Malaria Vaccine: Pfs25-rEPA
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