Poxvirus Assembly

传染性痘苗病毒颗粒有两种主要类型：成熟病毒颗粒（MV）和有包膜病毒颗粒（EV）。 MV 由被脂蛋白膜包围的 DNA 蛋白核心组成，在细胞质病毒工厂中组装，包含约 80 种多肽。 MV 亚群被修饰的反高尔基体或含有额外病毒膜蛋白的内体池包裹，沿着微管转运到细胞外周，并通过质膜离开细胞。 EV 本质上是具有额外膜的 MV，该膜在进入过程中与细胞融合之前被破坏。最具挑战性的问题之一是未成熟病毒粒子的初始病毒膜是如何形成的。通过生物化学、遗传学和显微镜方法，我们获得了内质网是病毒膜来源的证据，并鉴定了参与这一过程的几种病毒蛋白。

There are two major types of infectious vaccinia virus particles: mature virions (MVs) and enveloped virions (EVs). The MVs, which consist of a DNA-protein core surrounded by a lipoprotein membrane, are assembled in cytoplasmic viral factories and contain about 80 polypeptides. A subpopulation of MVs is wrapped by modified trans-Golgi or endosomal cisterna containing additional viral membrane proteins, transported along microtubules to the cell periphery, and exits the cell through the plasma membrane. The EVs are essentially MVs with an additional membrane that is disrupted prior to fusion of the MV with the cell during entry. One of the most challenging questions is how the initial viral membrane of the immature virion is formed. Using biochemical, genetic and microscopic approaches, we have obtained evidence that the endoplasmic reticulum is the source of the viral membrane and have identified several viral proteins involved in this process.