CYTOMETRY AND ANTIBODY TECHNOLOGY

项目总结（见说明）：细胞计数和抗体技术（CAT）设施为芝加哥大学综合癌症中心（UCCCC）会员提供测量细胞及其产物特性所需的所有工具。Fitch Monoclonal Subcore（FMS）开发用于标记细胞的试剂，而流式细胞术Subcore（FCS）提供使用这些试剂进行实验测量的技术和工作人员专业知识。在过去的35年中，抗体已成为基础和临床研究以及疾病诊断和治疗的不断发展的跨学科方法中可靠和必要的工具。抗体技术及其使用与流式细胞术和图像细胞术同时发展，并已成为细胞和细胞产物鉴定和分类的主要工具，特别是在癌症研究领域。UCCCC的研究人员在许多涉及细胞表型和功能的基础和转化研究的研究领域使用该设施。CAT设施的具体目标是提供仪器和抗体，用于使用一系列基于荧光和非荧光的检测技术对亚细胞成分和蛋白质进行分析检测。此外，计算机辅助分析设施的设计是为了满足对专门细胞计数分析的广泛需求，并继续满足对新技术和改进技术的需求。这些改进包括扩大抗体/杂交瘤库，为UCCCC研究人员提供常用的、最高质量的纯化抗体、用于流式细胞术的预滴定结合抗体和杂交瘤细胞系。细胞计数技术的改进包括最新的高速细胞分选、用于进行多色流式细胞术的多激光分析仪。基于图像的细胞仪和基于微珠的分析物检测器。

PROJECT SUMMARY (See instructions): The Cytometry and Antibody Technology (CAT) Facility serves the University of Chicago Comprehensive Cancer Center (UCCCC) membership by providing all of the tools necessary to measure properties of cells and their products. The Fitch Monoclonal Subcore (FMS) develops the reagents used to label the cells, whereas the Flow Cytometry Subcore (FCS) provides the technology and staff expertise to use these reagents for experimental measurements. During the past 35 years, antibodies have become reliable and necessary tools in the continuously growing interdisciplinary approach to basic and clinical research, as well as the diagnosis, and treatment of disease. Antibody technology and its usage have evolved concurrently with flow and image cytometry and has become the dominant tool for the identification and classification of cells and cellular products, especially in the areas of cancer research. The Facility is utilized by UCCCC investigators in many areas of study involved in the basic and translational research of cellular phenotype and function. The specific aim of the CAT Facility is to provide instrumentation and antibodies for the analytical detection of subcellular components and proteins using a range of fluorescence and non-fluorescence-based detection technologies. Further, the CAT Facility is designed to meet the wide-spread needs for specialized cytometric analysis and continues to respond to the demand for new and improved technology. Among these improvements include an expanding Antibody/Hybridoma Bank that provides commonly used, highest quality purified antibodies, pre-titered conjugated antibodies for flow cytometry, and hybridoma lines to UCCCC investigators. Cytometric technology improvements include the latest in high-speed cell sorting, multi-laser analyzers for performing polychromatic flow cytometry. Image-based cytometers, and bead-based analyte detectors.