Gene Expression and Microarrays

基因表达和微阵列

• 批准号: 8734185
• 负责人: Richard Paules
• 金额: $ 193.26万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8734185
• 关键词: Address; Bioinformatics; ChIP-on-chip; Computer software; Coupled; Custom; Development; Embryo; Ensure; Environment; Gene Chips; Gene Expression; Gene Expression Profiling; Genomics; Health; Housing; Human; Lasers; Messenger RNA; MicroRNAs; Microarray Analysis; Mission; Modification; National Institute of Environmental Health Sciences; Protocols documentation; RNA; Sampling; Scientist; Source; System; Technology; Testing; Tissues; chromatin immunoprecipitation; deep sequencing; genome-wide; human disease; improved; programs; research study

The mission of the NIEHS Microarray Core (NMC) is to utilize advances in genomic and bioinformatic technologies to enable Intramural NIEHS scientists to pursue studies that address how the environment influences human health. The NMC continuously strives to develop and implement new analytical and computational approaches to investigate genome-wide changes in gene expression. Specific technical accomplishments include testing and optimization of RNA isolation and amplification protocols to perform microarray experiments with RNA from limited tissue sources e.g. laser capture microdissected and embryonic samples. The NMC also incorporated the Illumina gene expression platform with our exiting Affymetrix and Agilent platforms and optimized the utilization of microarray technologies that extend the capacity to explore genome-wide analyses such as chromatin immunoprecipitation coupled with microarray chips (ChIP-chip) and microRNA (miRNA) applications. Additionally, efforts are underway to investigate the utilization of NextGen Deep Sequencing approaches for mRNA and miRNA expression analyses. The installation and implementation of software packages for emerging and existing genomic technologies has greatly improved the bioinformatics capacity of the NMC. In addition, continuing modifications to the NIEHS Microarray Core custom in-house MicroArray Project System (MAPS) have ensured that the NMC can sufficiently track and manage sample and experimental information related to microarray analyses. Finally, the NMC has continued to refine a rigorous QA/QC protocol incorporated into a standard deliverable that is distributed upon completion of a project.

NIEHS微阵列核心（NMC）的使命是利用基因组学和生物信息学技术的进步，使NIEHS内部科学家能够进行研究，解决环境如何影响人类健康。NMC不断努力开发和实施新的分析和计算方法，以研究基因表达的全基因组变化。具体的技术成就包括RNA分离和扩增方案的测试和优化，以使用来自有限组织来源的RNA进行微阵列实验，例如激光捕获显微切割和胚胎样品。NMC还将Illumina基因表达平台与我们现有的Affytek和Agilent平台相结合，并优化了微阵列技术的利用，这些技术扩展了探索全基因组分析的能力，例如染色质免疫沉淀与微阵列芯片（ChIP芯片）和microRNA（miRNA）应用。 此外，正在努力研究利用NextGen深度测序方法进行mRNA和miRNA表达分析。 为新兴和现有的基因组技术安装和实施软件包，大大提高了国家监测中心的生物信息学能力。此外，对NIEHS Microarray Core自定义内部MicroArray Project System（MAPS）的持续修改确保了NMC可以充分跟踪和管理与微阵列分析相关的样本和实验信息。最后，NMC继续完善严格的QA/QC协议，将其纳入项目完成后分发的标准可交付成果中。