Gene Expression and Microarrays

基因表达和微阵列

• 批准号: 7970096
• 负责人: Richard Paules
• 金额: $ 154.84万
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7970096
• 关键词: Address; Arts; Bioinformatics; Computer software; Coupled; Custom; DNA; Development; Embryo; Ensure; Environment; Gene Expression; Genomics; Health; Housing; Human; Lasers; MicroRNAs; Microarray Analysis; Mission; Modification; National Institute of Environmental Health Sciences; Protocols documentation; RNA; Sampling; Scientist; Source; System; Technology; Testing; Tissues; chromatin immunoprecipitation; genome-wide; human disease; improved; programs; research study

The mission of the NIEHS Microarray Core (NMC) is to utilize advances in genomic and bioinformatic technologies to enable Intramural NIEHS scientists to pursue studies that address how the environment influences human health. The NMC continuously strives to develop and implement new analytical and computational approaches to investigate genome-wide changes in gene expression. Specific technical accomplishments include testing and optimization of RNA isolation and amplification protocols to perform microarray experiments with RNA from limited tissue sources e.g laser capture microdissected and embryonic samples. The NMC also incorporated a new gene expression platform (Illumina) and optimized the utilization of microarray technologies that extend the capacity to explore genome-wide analyses such as chromatin immunoprecipitation coupled with microarray chips (ChIP-chip) and microRNA (miRNA) applications. The installation and implementation of software packages (e.g. Agilent DNA Analytics and Affymetrix GeneChip Command Console) for emerging and existing genomic technologies has greatly improved the bioinformatics capacity of the NMC. In addition, continuing modifications to the NIEHS Microarray Core custom in-house MicroArray Project System (MAPS) have ensured that the NMC can sufficiently track and manage sample and experimental information related to microarray analyses. Finally, the NMC has continued to refine a rigorous QA/QC protocol incorporated into a standard deliverable that is distributed upon completion of a project.

NIEHS微阵列核心（NMC）的使命是利用基因组和生物信息学技术的进步，使NIEHS的内部科学家能够从事环境如何影响人类健康的研究。NMC不断努力开发和实施新的分析和计算方法来研究基因表达的全基因组变化。具体的技术成就包括测试和优化RNA分离和扩增协议，以便对来自有限组织来源的RNA进行微阵列实验，例如激光捕获微解剖和胚胎样本。NMC还整合了一个新的基因表达平台（Illumina），并优化了微阵列技术的利用，扩展了探索全基因组分析的能力，如染色质免疫沉淀结合微阵列芯片（ChIP-chip）和microRNA （miRNA）应用。安装和实施用于新兴和现有基因组技术的软件包（例如安捷伦DNA分析和Affymetrix基因芯片命令控制台）大大提高了NMC的生物信息学能力。此外，NIEHS微阵列核心定制内部微阵列项目系统（MAPS）的持续修改确保了NMC能够充分跟踪和管理与微阵列分析相关的样品和实验信息。最后，NMC继续完善严格的QA/QC协议，并将其纳入项目完成后分发的标准可交付成果中。