Stable Isotope Resolved Metabolomics Analytical Shared Core

项目总结（见说明）：稳定同位素解析（SIRM）分析共享核心将为这些小组提供高信息通量（HIT）生物分析支持，特别强调基于稳定同位素的代谢途径追踪。该核心的基础已经以NSF发起的UofL监管和环境分析代谢组学中心（CREAM）的形式存在，该中心自2002年以来专门为SIRM代谢组学生物分析服务以及样品制备和处理，高场NMR，质谱和生物化学信息学方面的合作而建立。CREAM拥有最先进的仪器，对几个研究小组的实验设计产生了深远的影响。因此，许多核心互动本质上是长期的协作努力。SIRM共享核心的主要目标是为所有三个研究项目提供一套共享资源，并无缝集成实验设计，数据收集和数据分析，涵盖组织培养，小鼠模型和人类受试者。核心将提供实验方法的组合，以确定癌性和非癌性肺细胞和组织的代谢表型，使用我们最先进的NMR和质谱分析平台。在与各个项目负责人协商后，将根据各个项目的需要量身定制稳定同位素分析的具体实验设计，但在各个项目之间保持一致，以便利综合分析。我们有三个具体目标来实现我们的目标：具体目标1。提供基础设施和专家样本处理，确保所有三个研究项目中样本的一致处理、加工和存档;具体目标2。为三个研究项目提供广泛的NMR和MS技术的访问和专业知识：A）为稳健和一致的基于SIRM的分析实验设计提供仪器和专业知识，以及B）提供广泛的NMR和MS技术的专家数据收集和分析。具体目标3。为研究项目提供数据分析支持，包括数据、代谢途径和生化机制分析，包括：A）实施基于网络的数据简化、质量控制和数据分析支持平台; B）提供识别和定量代谢物的方法; C）在戈尔A的项目、数据采集和生化分析之间提供桥梁。核心B提供广泛的互操作性，处理和建议样品制备，数据收集，数据分析和质量控制的各个方面，确保所有3个研究项目的数据一致性。

PROJECT SUMMARY (See instructions): The Stable Isotope Resolved (SIRM) Analytical Shared Core will provide high information throughput (HIT) bioanalytical support for the groups, with special emphasis on stable isotope-based metabolic pathway tracing. The basis for this Core already exists in the form of the NSF-initiated, UofL Center for Regulatory and Environmental Analytical Metabolomics (CREAM), established expressly for SIRM metabolomic bioanalytical services and collaborations on sample preparation and handling, high field NMR, mass spectrometry, and biochemoinformatics since 2002. CREAM houses state of the art instrumentation that has profoundly influenced experimental design for several research groups. Therefore, many Core interactions are by their nature long-standing, collaborative efforts. The main goals of the SIRM Shared Core are to provide a set of shared resources for all three Research Projects and seamlessly integrate experimental design, data collection, and data analysis spanning tissue culture, mouse models and human subjects. The Core will provide a combination of experimental methods to determine metabolic phenotypes of cancerous and non-cancerous lung cells and tissue using our state of the art analytical platforms in both NMR and mass spectrometry. In consultation with individual project leaders, specific experimental design for stable isotope analysis will be tailored to individual project needs, but with consistency across projects to facilitate integrated analyses. We have 3 specific aims to achieve our goals: Specific Aim 1. Provide infrastructure and expert sample handling that ensures consistent handling, processing, and archiving of samples across all three Research Projects; Specific Aim 2. Provide access and expertise across a wide range of NMR and MS technologies to the three Research Projects: A) Provide access to instrumentation and expertise for robust and consistent SIRM-based analytical experimental design and B) Provide expert data collection and analysis across a wide range of NMR and MS technologies. Specific Aim 3. Provide data analytical support to the Research Projects, including data, metabolic pathway, and biochemical mechanism analyses, that include: A) Implement a web-based platform for data reduction, quality control and data analytical support; B) Provide methodologies for identifying and quantifying metabolites; C) Provide a bridge between the Projects, data acquisition and biochemical analysis in Gore A. Core B provides extensive interoperability, handling and advising all aspects of sample preparation, data collection, data analysis & quality control, ensuring consistency of data across all 3 research projects.