Resource Center for Stable Isotope-Resolved Metabolomics

稳定同位素解析代谢组学资源中心

基本信息

批准号：
8916696
负责人：
Teresa Whei-Mei Fan
金额：
$ 200万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-11 至 2016-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8916696
关键词：
Address Adoption Algorithms Animal Model Animals Arts Award Biochemical Pathway Biomedical Research Cardiovascular Diagnostic Techniques Cell Culture Techniques Cells Client Clinical Research Communities Consultations Data Data Analyses Detection Development Devices Diabetes Mellitus Disease Distance Learning Education and Outreach Educational workshop Ensure Experimental Designs Fees Foundations Fractionation Freezing Fresh Tissue Funding Goals Grant Homeostasis Human Human Genome Informatics Infusion procedures Instruction International Internet Joints Laboratories Laboratory Research Lead Leadership Learning Malignant Neoplasms Mass Spectrum Analysis Metabolism Methodology Microfluidics Modeling Neurodegenerative Disorders Outcome Paper Pathway interactions Performance Preparation Procedures Process Proteome Publications Publishing Qualifying Regulation Research Research Personnel Resected Resource Informatics Resources Sampling Services Slice Solid Structure System Techniques Technology Therapeutic Tissues Tracer Training Training and Education Transplantation United States National Institutes of Health Universities Work Xenograft procedure base bench to bedside computerized data processing cost data acquisition design disease diagnosis experience handbook human subject instrumentation meetings metabolomics network models new therapeutic target operation outreach prevent programs software development stable isotope symposium tandem mass spectrometry therapeutic development ultra high resolution

项目摘要

DESCRIPTION: The human metabolome represents the functional display of the human genome and proteome. The regulation of its homeostasis is highly sensitive to both intrinsic and external factors, the perturbation of which can lead to disease development. Advances in mass spectrometry (MS), NMR, informatics, and sample processing have made possible large-scale interrogation of the human metabolome ("metabolomics") and is being increasingly applied to a variety of diseases, such as cancer, diabetes, neurodegenerative and cardiovascular disorders, for diagnosis and therapeutic purposes. The existing Center for Regulatory and Environmental Analytical Metabolomics (CREAM) conducts training and provides advice and full analytical resources, from sample processing, MS and NMR instrumentation, to informatics, which match with the major goals of the Common Fund. Moreover, CREAM is now in its 6th year of self- sufficiency through client fees and conducts translational clinical research, both of which are critical fifth-year exit goals of the Common Fund. Building on this solid foundation with added resources, the proposed Common Fund Regional Comprehensive Metabolomics Resource Core (RCMRC) at the University of Louisville will greatly expand CREAM's capabilities in sample processing, analytical/informatics developments and services, and outreach efforts under the leadership of the PD and Pis, who collectively have more than 95 years of experience in metabolomics. The Overall Goals of the RCMRC-CREAM are to facilitate and promote metabolomics research with an emphasis on stable isotope tracing for pathway elucidation in studies ranging from laboratory bench cell cultures, model animals, to human subjects, and to provide the state-of-art analytical and informatics resources, education, and training for the biomedical research community. These goals will be achieved via the following specific aims: SA1: To administer comprehensive Metabolomics resources for bench to bedside research; SA2: To develop and implement integrated Metabolomics platforms to enhance performance, sample & information throughput, and scientific relevance; SA3: To provide outreach and education of the wider community in Metabolomics approaches.

描述：人代谢组代表人类基因组和蛋白质组的功能表现。其体内平衡的调节对内在因素和外部因素都高度敏感，其扰动会导致疾病发展。质谱（MS），NMR，信息学和样本处理的进步使对人代谢组（“代谢组学”）的大规模询问，并且越来越多地应用于多种疾病，例如癌症，糖尿病，神经减退性和心血管疾病，以便诊断和诊断。现有的监管和环境分析代谢组学中心（CRAM）进行培训，并提供咨询和完整的分析资源，从样本处理，MS和NMR仪器到信息学，这些资源与共同基金的主要目标相匹配。此外，Cream现在正处于客户费用的第六年，并通过客户费用进行转化临床研究，这两者都是普通基金的五年级退出目标。拟议中的基础资源以扎实的基础为基础，在路易斯维尔大学（University of Louisville）的普通基金区域综合代谢组学资源核心（RCMRC）将大大扩展乳霜在样本处理，分析/信息信息发展和服务方面的能力，以及在PD和PIS的领导下进行宣传工作，他们在PIS的领导下，他们在95年以上具有超过95年的代理经验。 RCMRC-Cream的总体目标是促进和促进代谢组学研究，重点是稳定的同位素追踪，以阐明途径，以阐明途径，从实验室基准细胞培养物，模型动物，人类受试者到人类受试者，并为生物学研究群体提供最先进的分析和信息资源，并提供最先进的分析和信息资源。这些目标将通过以下特定目的实现：SA1：管理综合代谢组学资源，用于卧床研究； SA2：开发和实施集成的代谢组学平台，以增强性能，样本和信息吞吐量以及科学相关性； SA3：在代谢组学方法中提供更广泛社区的外展和教育。