Resource Center for Stable Isotope-Resolved Metabolomics

人类代谢组代表了人类基因组和蛋白质组的功能展示。其体内平衡的调节对内在和外部因素都高度敏感，其扰动可导致疾病的发展。质谱（MS）、核磁共振、信息学和样品处理方面的进步使得对人类代谢组学（“代谢组学”）的大规模研究成为可能，并越来越多地应用于各种疾病，如癌症、糖尿病、神经退行性疾病和心血管疾病，用于诊断和治疗目的。现有的监管和环境分析代谢组学中心（CREAM）进行培训，并提供建议和完整的分析资源，从样品处理、质谱和核磁共振仪器到信息学，这些都与共同基金的主要目标相匹配。此外，CREAM现在已经是第六年通过客户费用自给自足，并进行转化临床研究，这两个都是共同基金第五个关键的退出目标。在这个坚实的基础上加上额外的资源，路易斯维尔大学拟议的共同基金区域综合代谢组学资源核心（RCMRC）将极大地扩展CREAM在样品处理、分析/信息学开发和服务方面的能力，并在PD和Pis的领导下开展外联工作，他们在代谢组学方面总共有超过95年的经验。RCMRC-CREAM的总体目标是促进和促进代谢组学研究，重点是稳定同位素示踪，以阐明从实验室细胞培养、模型动物到人类受试者的研究，并为生物医学研究界提供最先进的分析和信息学资源、教育和培训。这些目标将通过以下具体目标实现：SA1：管理从实验室到床边研究的综合代谢组学资源；SA2：开发和实施集成代谢组学平台，以提高性能，样品和信息吞吐量以及科学相关性；SA3：向更广泛的社区提供代谢组学方法的推广和教育。

DESCRIPTION: The human metabolome represents the functional display of the human genome and proteome. The regulation of its homeostasis is highly sensitive to both intrinsic and external factors, the perturbation of which can lead to disease development. Advances in mass spectrometry (MS), NMR, informatics, and sample processing have made possible large-scale interrogation of the human metabolome ("metabolomics") and is being increasingly applied to a variety of diseases, such as cancer, diabetes, neurodegenerative and cardiovascular disorders, for diagnosis and therapeutic purposes. The existing Center for Regulatory and Environmental Analytical Metabolomics (CREAM) conducts training and provides advice and full analytical resources, from sample processing, MS and NMR instrumentation, to informatics, which match with the major goals of the Common Fund. Moreover, CREAM is now in its 6th year of self- sufficiency through client fees and conducts translational clinical research, both of which are critical fifth-year exit goals of the Common Fund. Building on this solid foundation with added resources, the proposed Common Fund Regional Comprehensive Metabolomics Resource Core (RCMRC) at the University of Louisville will greatly expand CREAM's capabilities in sample processing, analytical/informatics developments and services, and outreach efforts under the leadership of the PD and Pis, who collectively have more than 95 years of experience in metabolomics. The Overall Goals of the RCMRC-CREAM are to facilitate and promote metabolomics research with an emphasis on stable isotope tracing for pathway elucidation in studies ranging from laboratory bench cell cultures, model animals, to human subjects, and to provide the state-of-art analytical and informatics resources, education, and training for the biomedical research community. These goals will be achieved via the following specific aims: SA1: To administer comprehensive Metabolomics resources for bench to bedside research; SA2: To develop and implement integrated Metabolomics platforms to enhance performance, sample & information throughput, and scientific relevance; SA3: To provide outreach and education of the wider community in Metabolomics approaches.