Resource Center for Stable Isotope-Resolved Metabolomics

描述：人类代谢组代表了人类基因组和蛋白质组的功能展示。其动态平衡的调节对内因和外因都高度敏感，外因的扰动可导致疾病的发展。质谱学、核磁共振、信息学和样品处理技术的进步使大规模的人类代谢组(代谢组学)研究成为可能，并越来越多地应用于各种疾病，如癌症、糖尿病、神经退行性疾病和心血管疾病，用于诊断和治疗目的。现有的监管和环境分析代谢学中心(CREAM)进行培训，提供咨询和全面的分析资源，从样品处理、MS和核磁共振仪器到信息学，符合共同基金的主要目标。此外，通过收取客户费用和进行转化性临床研究，CREAM目前已进入自给自足的第六个年头，这两项都是共同基金第五年的关键退出目标。在这个坚实的基础上，通过增加资源，路易斯维尔大学拟议的共同基金区域综合代谢组学资源核心(RCMRC)将在PD和PIS的领导下极大地扩展CREAM在样品处理、分析/信息学开发和服务以及推广工作方面的能力，这两个人在代谢组方面总共拥有超过95年的经验。RCMRC-CREAM的总体目标是促进和促进代谢组学研究，重点是稳定的同位素追踪，以在从实验室长凳细胞培养、模型动物到人类受试者的研究中阐明途径，并为生物医学研究界提供最先进的分析和信息学资源、教育和培训。这些目标将通过以下具体目标实现：SA1：管理全面的代谢组学资源，用于从临床到床边的研究；SA2：开发和实施综合代谢组学平台，以提高绩效、样本和信息吞吐量以及科学相关性；SA3：在代谢组学方法方面向更广泛的社区提供外联和教育。

DESCRIPTION: The human metabolome represents the functional display of the human genome and proteome. The regulation of its homeostasis is highly sensitive to both intrinsic and external factors, the perturbation of which can lead to disease development. Advances in mass spectrometry (MS), NMR, informatics, and sample processing have made possible large-scale interrogation of the human metabolome ("metabolomics") and is being increasingly applied to a variety of diseases, such as cancer, diabetes, neurodegenerative and cardiovascular disorders, for diagnosis and therapeutic purposes. The existing Center for Regulatory and Environmental Analytical Metabolomics (CREAM) conducts training and provides advice and full analytical resources, from sample processing, MS and NMR instrumentation, to informatics, which match with the major goals of the Common Fund. Moreover, CREAM is now in its 6th year of self- sufficiency through client fees and conducts translational clinical research, both of which are critical fifth-year exit goals of the Common Fund. Building on this solid foundation with added resources, the proposed Common Fund Regional Comprehensive Metabolomics Resource Core (RCMRC) at the University of Louisville will greatly expand CREAM's capabilities in sample processing, analytical/informatics developments and services, and outreach efforts under the leadership of the PD and Pis, who collectively have more than 95 years of experience in metabolomics. The Overall Goals of the RCMRC-CREAM are to facilitate and promote metabolomics research with an emphasis on stable isotope tracing for pathway elucidation in studies ranging from laboratory bench cell cultures, model animals, to human subjects, and to provide the state-of-art analytical and informatics resources, education, and training for the biomedical research community. These goals will be achieved via the following specific aims: SA1: To administer comprehensive Metabolomics resources for bench to bedside research; SA2: To develop and implement integrated Metabolomics platforms to enhance performance, sample & information throughput, and scientific relevance; SA3: To provide outreach and education of the wider community in Metabolomics approaches.