Remote focusing through spatial light modulation for multifocal multiphoton micro

通过空间光调制进行远程聚焦，实现多焦点多光子微

• 批准号: 8454181
• 负责人: JAY E STOCKLEY
• 金额: $ 9.8万
• 依托单位: BOULDER NONLINEAR SYSTEMS, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-08 至 2014-08-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8454181
• 关键词: Address; Biological; Communities; Detection; Development; Elements; Figs - dietary; Fluorescence; Generations; Image; Imaging technology; Lasers; Lateral; Life; Light; Location; Mechanics; Medical; Microscope; Microscopy; Neurologic; Neurosciences; Optics; Organism; Phase; Physiologic pulse; Positioning Attribute; Research Personnel; Resolution; Sampling; Scanning; Scheme; Shapes; Specimen; System; Techniques; Testing; Time; Tissues; arm; base; biological systems; cost; cost effective; design; detector; flexibility; imaging probe; improved; in vivo; liquid crystal; prevent; public health relevance; two-photon; user-friendly

DESCRIPTION (provided by applicant): Nonlinear optical microscopy techniques (such as two-photon florescence) are being used to acquire volumetric (i.e. three dimensional) images that probe several hundred microns into scattering tissue. These techniques are being combined with fast acquisition schemes to allow imaging of live, moving specimens at high NA. This combination allows for high-resolution, in vivo exploration of biological specimens. However, the added system complexity prevents these microcopy techniques from being readily exploited by the biological and medical communities that would greatly benefit from them. Therefore, we propose the development of a simplified confocal microscope configuration that rapidly acquires volumetric images within a scattering media. It uses cost-effective and user-friendly components, meaning they do not require optical, electrical or mechanical expertise to setup and operate.

描述（由申请人提供）：非线性光学显微镜技术（例如双光子荧光）正在用于获取体积（即三维）图像，这些图像可以探测数百微米的散射组织。这些技术正在与快速采集方案相结合，以允许在高NA下对活的、移动的标本进行成像。这种组合允许生物样本的高分辨率体内探索。然而，增加的系统复杂性阻止了这些微复制技术被生物和医学界容易地利用，这将大大受益于它们。因此，我们提出了一个简化的共聚焦显微镜配置，快速获取体积内的散射介质中的图像的发展。它使用具有成本效益和用户友好的组件，这意味着它们不需要光学，电气或机械专业知识来设置和操作。