Biotechnology and Bioinformatics Core

(核心负责人：张兵)生物技术和生物信息学核心提供RNA-Seq数据生成、分析、解释和共享服务。这些服务由尖端的实验和 哈德逊阿尔法理工学院的计算基础设施(其中RNA-Seq数据 将进行生成和低级别数据分析)和范德比尔特大学(高级别 将进行RNA-Seq数据分析、数据解释和共享)。哈德逊阿尔法研究所 10台Illumina HiSeq测序仪及其在Illumina系统上进行下一代测序的能力 每年超过3200条车道。它还拥有一个集中的计算基础设施，其中包括256- 核心群集，RAID5格式的快速磁盘存储中具有640TB的可用空间(超过总存储容量的1PB)。 Vanderbilt计算基础设施包括一个高性能计算集群，该集群具有大约 3,000个处理器核心，理论峰值性能为12万亿次浮点运算。该文件系统提供 超过200TB的磁盘空间，144 GB的磁盘缓存，可以为集群提供60 GB/S的磁盘带宽。 HudsonAlpha和Vanderbilt都是Internet2高级技术社区的成员，允许 以极快的速度传输将在拟议项目中生成的超大数据集 这些机构。生物信息学和计算工具，如Perl、BioPeri、R、BioConductor、MySQL、 BioMart、FASTX-TOOLKIT、SamTools、The

(Core Leader: Bing Zhang) The Biotechnology and Bioinformatics Core provides service in RNA-Seq data generation, analysis, Interpretation, and sharing. These services are powered by cutting edge experimental and computational infrastructures at the HudsonAlpha Institute of Technology (where the RNA-Seq data generation and low-level data analysis will be performed) and the Vanderbilt University (where high-level RNA-Seq data analysis, data interpretation and sharing will be performed). The HudsonAlpha Institute has ten Illumina HiSeq sequencing instruments, and its capacity for next-gen sequencing on the Illumina systems is more than 3,200 lanes per year. It also has a centralized computational infrastructure that includes a 256- core cluster with 640TB of available space in fast disk storage in RAID5 format (over 1PB of total storage). The Vanderbilt computational infrastructure includes a high performance computing cluster that has about 3,000 processor cores and has a theoretical peak performance of 12 TeraFLOPS. The file system provides over 200TB of disk space, 144GB of disk cache, and can sustain 60Gb/s of disk bandwidth to the cluster. Both HudsonAlpha and Vanderbilt are members of the Internet2 advanced technology community, allowing extremely rapid transfer of the very large datasets that will be generated in the proposed projects between the institutions. Bioinformatics and Computational tools such as Perl, BioPeri, R, BioConductor, MySQL, BioMart, FASTX-Toolkit, SAMtools, the